FITC-Linked Polyclonal Antibody to Cytochrome P450 27B1 (CYP27B1)

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Properties

If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
Conjugation

Anti-FITC Antibody
Group

Polyclonals and antibodies
About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
French translation

anticorps

Gene target

Linked Cytochrome P450 27B1 CYP27B1
Gene symbol

CYP3A7, CYP27B1, CYP24A1, CYP1A1, CYP51A1, CYP2D6
Short name

FITC-Linked Polyclonal Antibody Cytochrome P450 27B1 (CYP27B1)
Technique

Polyclonal, Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
Label

FITC
Alternative name

fluorecein-Linked polyclonal (antibody to-) to Cytochrome P450 27B1 (cytochrome P450, family 27, subfamily B, polypeptide 1)
Alternative technique

polyclonals, antibodies, fluorescine
Alternative to gene target

cytochrome P450, family 27, subfamily B, polypeptide 1, CP2B and CYP1 and CYP1alpha and CYP27B and P450c1 and PDDR and VDD1 and VDDR and VDDRI and VDR, CYP27B1 and IDBG-43755 and ENSG00000111012 and 1594, oxidoreductase activity, Cytoplasm, Cyp27b1 and IDBG-194013 and ENSMUSG00000006724 and 13115, BT.61430 and IDBG-634881 and ENSBTAG00000016906 and 539630

Identity

HGNC:2640
Gene

CYP3A7
Long gene name

cytochrome P450 family 3 subfamily A member 7
Synonyms gene name
- cytochrome P450, subfamily IIIA, polypeptide 7
- cytochrome P450, family 3, subfamily A, polypeptide 7
Synonyms
- CP37
- P450-HFLA
GenBank acession
- AF315325
Locus

7q22.1
Discovery year

1992-09-14
Entrez gene record

1551
Pubmed identfication
- 2722762
Classification
- Cytochrome P450 family 3
VEGA ID

OTTHUMG00000156726
Locus Specific Databases
- PharmVar Human Cytochrome P450 (CYP) Allele Nomenclature

Identity

HGNC:2606
Gene

CYP27B1
Long gene name

cytochrome P450 family 27 subfamily B member 1
Synonyms gene
- VDD1
- PDDR
Synonyms gene name
- cytochrome P450, subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase), polypeptide 1
- cytochrome P450, family 27, subfamily B, polypeptide 1
Synonyms
- CYP1
- P450c1
Synonyms name
GenBank acession
- AB006987
Locus

12q14.1
Discovery year

1998-03-24
Entrez gene record

1594
Pubmed identfication
- 9295274
- 9344864
RefSeq identity
- NM_000785
Classification
- Cytochrome P450 family 27
VEGA ID

OTTHUMG00000170457

Identity

HGNC:2602
Gene

CYP24A1
Long gene name

cytochrome P450 family 24 subfamily A member 1
Synonyms gene
- CYP24
Synonyms gene name
- cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)
- cytochrome P450, family 24, subfamily A, polypeptide 1
Synonyms
- CP24
- P450-CC24
GenBank acession
- U60669
Locus

20q13.2
Discovery year

1992-09-09
Entrez gene record

1591
Classification
- Cytochrome P450 family 24
VEGA ID

OTTHUMG00000032773

Identity

HGNC:2595
Gene

CYP1A1
Long gene name

cytochrome P450 family 1 subfamily A member 1
Synonyms gene
- CYP1
Synonyms gene name
- cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1
- cytochrome P450, family 1, subfamily A, polypeptide 1
Synonyms
- P450DX
- P1-450
- P450-C
- CP11
GenBank acession
- BC023019
Locus

15q24.1
Discovery year

1986-01-01
Entrez gene record

1543
Pubmed identfication
- 15128046
RefSeq identity
- NM_000499
Classification
- Cytochrome P450 family 1
VEGA ID

OTTHUMG00000142812
Locus Specific Databases
- PharmVar Human Cytochrome P450 (CYP) Allele Nomenclature
- LRG_1262

Identity

HGNC:2649
Gene

CYP51A1
Long gene name

cytochrome P450 family 51 subfamily A member 1
Synonyms gene
- CYP51
Synonyms gene name
- cytochrome P450, 51 (lanosterol 14-alpha-demethylase)
- cytochrome P450, family 51, subfamily A, polypeptide 1
Synonyms
- CP51
- CYPL1
- P450L1
- LDM
- P450-14DM
GenBank acession
- U51685
Locus

7q21.2
Discovery year

1996-10-26
Entrez gene record

1595
Pubmed identfication
- 8975714
Classification
- Cytochrome P450 family 51
VEGA ID

OTTHUMG00000131131

Name

Fluorescent Antibody Technique
Concept

Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data