PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx

  • Catalog number
    31051-X
  • Price
    Please ask
  • Size
    1 kit
  • Description
    PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx
  • Category
    PCR and related reagents
  • Shipping temperature
    2°C to 8°C
  • Shipping conditions
    blue ice
  • Storage
    -30°C to -2°C
  • Hazard pictogram
    N/A
  • Signal word
    N/A
  • Hazard statements
    N/A
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield. Real-time PCR amplification mixes are available for quantification with specific selected primers and with optimized buffers.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Additional description
    Real time PCR kits are used with DNA extraction kits supplied in 50 or 100 tests with polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR quantitatively (Quantitative real-time PCR), semi-quantitatively. RNA is often converted to cDNA. Often used as diagnostic tool. Ct values will have to be set for your probes.
  • Gene target
  • Short name
    PMA Real-Time PCR Bacterial Viability Kit - Listeria monocytogenes (hly) PMAxx
  • Technique
    Real-time, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
  • Species
    Listeria, Listeria
  • Alternative name
    PMA Real-Time PCR test kit Bacterial cytotoxity reagent - Listeria monocytogenes (hly) PMAxx
  • Alternative technique
    rtpcr, kits, dna-amplification
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Disease
    Listeria monocytogenes and Listeria species are gram-positive, rod-shaped, facultatively anaerobic, and non spore-forming. The major human pathogen in the Listeria genus is L. monocytogenes. It is usually the causative agent of the relatively rare bacterial disease listeriotic, listeriosis a serious infection caused by eating food contaminated with the bacteria. The disease affects pregnant women, newborns, adults with weakened immune systems, and the elderly. Antibodies and ELISA test kits for detection of listeria antigens.
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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