pLentG-KOSM Lentiviral Vector

  • Catalog number
    LTV-700
  • Price
    Please ask
  • Size
    100 µL
  • We send this reagant on
    Blue Ice
  • The temperatureerature for storage is
    -80ºC
  • SDS or safty data sheet
    more (LTV-700) GHS.pdf
  • Type of reagent
    Stem Cell Research
  • Related techniques
    Stem Cell Research, iPS Cell Reprogramming, Lentiviral Vectors for iPS Cell Generation
  • Use of the reagent
    pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. Producing iPS cells using pLentG-KOSM not only provides higher efficiency than other methods, but also reduces time and reagent cost by allowing you to package a single lentivirus rather than several viruses each containing a single gene.
  • Cellular use
    Single polycistronic vector contains fusion of mouse Klf4, Oct3/4, Sox2 and c-Myc genes
  • Protocol information
    Efficiency of iPS cell generation from single polycistronic virus is >10-fold higher efficiency than multi-virus transduction and >500-fold higher than non-viral methods
  • Reagent properties
    Also contains GFP reporter
  • Living modified organisme in this reagent or LMO
    E.coli Unfiltered
  • Conjugation and form
    host specific
  • Species compatible to
    human cells and cell lines and other cells
  • Use of this test kit or product
    Molecular biology
  • Antigen
    the sequence of the protein
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,87
  • Molecular weight
    kiloDalton
  • This reagent is purified
    0.98
  • Gene target
  • Short name
    pLentG-KOSM Lentiviral Vector
  • Technique
    Vectors
  • Alternative name
    pLentG-KOSM Lentiviral integrating Desoxyribonucleic acid sequence
MeSH Data
  • Name
  • Concept
    Scope note: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
  • Tree numbers
    • E05.393.220.250
  • Qualifiers
    drug effects, methods, radiation effects
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