pLentG-KOSM Lentiviral Vector
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Catalog number
LTV-700
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Price
Please ask
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Size
100 µL
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
-80ºC
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SDS or safty data sheet
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Type of reagent
Stem Cell Research
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Related techniques
Stem Cell Research, iPS Cell Reprogramming, Lentiviral Vectors for iPS Cell Generation
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Use of the reagent
pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. Producing iPS cells using pLentG-KOSM not only provides higher efficiency than other methods, but also reduces time and reagent cost by allowing you to package a single lentivirus rather than several viruses each containing a single gene.
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Cellular use
Single polycistronic vector contains fusion of mouse Klf4, Oct3/4, Sox2 and c-Myc genes
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Protocol information
Efficiency of iPS cell generation from single polycistronic virus is >10-fold higher efficiency than multi-virus transduction and >500-fold higher than non-viral methods
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Reagent properties
Also contains GFP reporter
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Living modified organisme in this reagent or LMO
E.coli Unfiltered
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,87
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Molecular weight
kiloDalton
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This reagent is purified
0.98
MeSH Data
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