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Available ordering format
Lyophilized
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Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PLD alpha 1, UniProt: Q38882, TAIR: AT3G15730 and PLD alpha 2, UniProt: Q9SSQ9, TAIR: AT1G52570
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
AS09 556 | PLD | phospholipase D, rabbit antibody to native enzyme from Brassica oleraceaPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent
91.8 (PLD alpha 1 precursor), 91.5 (PLD alpha 2 precursor)
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Verified reactivity
Arabidopsis thaliana, Brassica oleracea
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Possible reactivity
Brassica napus, Brassica rapa, Capsella rubella, Carica papaya, Gossypium hirsutum, Glycine max, Ricinus communis
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
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References
to be added when available, antibody released in November 2013.
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Scientific context
PLDA1/2 (phospholipase D alpha 1/2) is an enzyme which hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond and plays an important tole in processes as phytochormone action and response to stress. This protein is highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds. Induced by ABA, ethylene, heavy metal, cold, salt and osmotic stresses. Alternative names:PLDalpha1,PLD alpha 1,Choline phosphatase 1 PLDalpha, Phosphatidylcholine-hydrolyzing phospholipase D 1, Choline phosphatase 2, Phosphatidylcholine-hydrolyzing phospholipase D 2, PLDalpha2, PLD alpha 2.
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Notes
None
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Description
The PLDA1/2, phospholipase D alpha 1/2 is a α- or alpha protein sometimes glycoprotein present in blood.