Platinum Retroviral Expression System, Ecotropic

  • Catalog number
    VPK-300
  • Price
    Please ask
  • Size
    1 test kit
  • We send this reagant on
    Dry Ice
  • The temperatureerature for storage is
    Store all plasmids at -20C. For best results begin culture of Platinum cells upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
  • SDS or safty data sheet
    more (VPK-300 through VPK-308) GHS.pdf
  • Type of reagent
    Viral Expression
  • Related techniques
    Viral Expression, Retrovirus Expression, Retroviral Expression and Packaging, Platinum Retroviral Expression Systems
  • Use of the reagent
    Retroviral vectors are useful for delivering genes of interest into a target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging. Platinum Retroviral Expression Systems incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection. This system includes our exclusive Platinum-E Packaging cell line, which contains the gag and pol genes and an ecotropic envelope protein for viral packaging. Simply clone your gene of interest into the vector provided and transfect into the packaging cells.
  • Cellular use
    Achieve average titer of 10^7 infectious units/mL with transient transfection
  • Protocol information
    Produce ecotropic retrovirus which will only readily infect mouse and rat cells
  • Reagent properties
    Cloning capacity of pMXs-Neo expression vector = 3.8 kb
  • Additional lab advice
    Cloning capacity of pMXs-Puro expression vector = 4.4 kb
  • Living modified organisme in this reagent or LMO
    Human cells transduced with retroviral genes
  • Species compatible to
    Humans
  • Use of this test kit or product
    Genetics lab
  • Antigen
    a specific protein sequence
  • Buffer
    The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,75
  • Molecular weight
    in kilo Danlto or kDa
  • This reagent is purified
    0.97
MeSH Data
  • Name
  • Concept
    Scope note: Process of growing viruses in live animals, plants, or cultured cells.
  • Tree numbers
    • E01.370.225.875.970
    • E05.200.875.970
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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