Platinum-GP Retroviral Packaging Cell Line, Pantropic
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Catalog numberRV-103
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PricePlease ask
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Size1 vial
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We send this reagant onDry Ice
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The temperatureerature for storage isFor best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
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SDS or safty data sheetmore (RV-101, 102, 103) GHS.pdf
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Type of reagentViral Expression
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Related techniquesViral Expression, Retroviral Expression, Retroviral Expression and Packaging, Platinum Retroviral Packaging Cell Lines
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Use of the reagentConventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.
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Cellular useHigher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection
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Protocol informationLonger stability: up to 4 months in the presence of drug selection
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Reagent propertiesProduce VSVG-pseudotyped pantropic retrovirus, which can easily infect cells of nearly any species
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Living modified organisme in this reagent or LMOHuman cells transduced with retroviral genes
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Conjugation and formhost specific
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Species compatible tohuman cells and cell lines and other cells
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Use of this test kit or productMolecular biology
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Antigenthe sequence of the protein
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BufferThe concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,41
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Molecular weightkiloDalton
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This reagent is purified0.98
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolADAM7, RNF130, GTPBP1, DMBT1, ABCB1
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Short namePlatinum-GP Retroviral Packaging Line, Pantropic
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Alternative namePlatinum-GP Retroviral Packaging cellular Line, Pantropic
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Tissuecell
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Gene info
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Identity
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Gene
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Long gene nameADAM metallopeptidase domain 7
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Synonyms gene name
- a disintegrin and metalloproteinase domain 7
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ADAM metallopeptidase domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namering finger protein 130
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-05-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
- Ring finger proteins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameGTP binding protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-04-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namedeleted in malignant brain tumors 1
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Synonyms
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Synonyms name
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Locus
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Discovery year1997-09-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Scavenger receptor cysteine rich domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameATP binding cassette subfamily B member 1
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Synonyms gene
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Synonyms gene name
- colchicin sensitivity
- ATP-binding cassette, sub-family B (MDR/TAP), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ATP binding cassette subfamily B
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Process of growing viruses in live animals, plants, or cultured cells.
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Tree numbers
- E01.370.225.875.970
- E05.200.875.970
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data