Platinum-GP Retroviral Packaging Cell Line, Pantropic

• Catalog number
  RV-103
• Price
  Please ask
• Size
  1 vial

• We send this reagant on
  Dry Ice
• The temperatureerature for storage is
  For best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
• Technical file
• SDS or safty data sheet
  more (RV-101, 102, 103) GHS.pdf
• Type of reagent
  Viral Expression
• Related techniques
  Viral Expression, Retroviral Expression, Retroviral Expression and Packaging, Platinum Retroviral Packaging Cell Lines
• Use of the reagent
  Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.
• Cellular use
  Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection
• Protocol information
  Longer stability: up to 4 months in the presence of drug selection
• Reagent properties
  Produce VSVG-pseudotyped pantropic retrovirus, which can easily infect cells of nearly any species
• Living modified organisme in this reagent or LMO
  Human cells transduced with retroviral genes
• Conjugation and form
  host specific
• Species compatible to
  human cells and cell lines and other cells
• Use of this test kit or product
  Molecular biology
• Antigen
  the sequence of the protein
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,41
• Molecular weight
  kiloDalton
• This reagent is purified
  0.98
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Platinum-GP   Retroviral   Packaging   Line   Pantropic  
• Gene symbol
  ADAM7, RNF130, GTPBP1, DMBT1, ABCB1
• Short name
  Platinum-GP Retroviral Packaging Line, Pantropic
• Alternative name
  Platinum-GP Retroviral Packaging cellular Line, Pantropic
• Tissue
  cell

Gene info

• Identity
  HGNC:214
• Gene
  ADAM7
• Long gene name
  ADAM metallopeptidase domain 7
• Synonyms gene name
  • a disintegrin and metalloproteinase domain 7
• Synonyms
  • GP-83
  • EAPI
• GenBank acession
  • AF090327
• Locus
  8p21.2
• Discovery year
  1998-12-01
• Entrez gene record
  8756
• Pubmed identfication
  • 15883027
  • 19855936
• RefSeq identity
  • NM_003817
• Classification
  • ADAM metallopeptidase domain containing
• VEGA ID
  OTTHUMG00000097859

Gene info

• Identity
  HGNC:18280
• Gene
  RNF130
• Long gene name
  ring finger protein 130
• Synonyms
  • GP
  • G1RZFP
  • GOLIATH
• GenBank acession
  • AF155650
• Locus
  5q35.3
• Discovery year
  2003-05-21
• Entrez gene record
  55819
• Pubmed identfication
  • 10806348
• RefSeq identity
  • NM_018434
• Classification
  • MicroRNA protein coding host genes
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000130909

Gene info

• Identity
  HGNC:4669
• Gene
  GTPBP1
• Long gene name
  GTP binding protein 1
• Synonyms
  • GP-1
  • HSPC018
• GenBank acession
  • U87964
• Locus
  22q13.1
• Discovery year
  1999-04-22
• Entrez gene record
  9567
• Pubmed identfication
  • 9070279
• RefSeq identity
  • NM_004286
• VEGA ID
  OTTHUMG00000151002

Gene info

• Identity
  HGNC:2926
• Gene
  DMBT1
• Long gene name
  deleted in malignant brain tumors 1
• Synonyms
  • GP340
  • muclin
  • SALSA
  • Gp-340
  • hensin
  • vomeroglandin
• Synonyms name
  • salivary agglutinin
  • salivary scavenger and agglutinin
• Locus
  10q26.13
• Discovery year
  1997-09-05
• Entrez gene record
  1755
• Pubmed identfication
  • 9288095
  • 17548659
• RefSeq identity
  • NM_004406
• Classification
  • Scavenger receptor cysteine rich domain containing
• VEGA ID
  OTTHUMG00000019185

Gene info

• Identity
  HGNC:40
• Gene
  ABCB1
• Long gene name
  ATP binding cassette subfamily B member 1
• Synonyms gene
  • PGY1
  • MDR1
  • CLCS
• Synonyms gene name
  • colchicin sensitivity
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Synonyms
  • P-gp
  • CD243
  • GP170
  • ABC20
  • p-170
• Synonyms name
  • multidrug resistance protein 1
• GenBank acession
  • M14758
• Locus
  7q21.12
• Discovery year
  1986-01-01
• Entrez gene record
  5243
• Pubmed identfication
  • 3027054
• RefSeq identity
  • NM_000927
• Classification
  • ATP binding cassette subfamily B
  • CD molecules
• VEGA ID
  OTTHUMG00000023393

MeSH Data

• Name
  Virus Cultivation
• Concept
  Scope note: Process of growing viruses in live animals, plants, or cultured cells.
• Tree numbers
  • E01.370.225.875.970
  • E05.200.875.970
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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