Platinum-A Retroviral Packaging Cell Line, Amphotropic

• Catalog number
  RV-102
• Price
  Please ask
• Size
  1 vial

• We send this reagant on
  Dry Ice
• The temperatureerature for storage is
  For best results begin culture upon receipt. If this is not possible, store at -80C until first culture. Store subsequent cultured cells in liquid nitrogen.
• Technical file
• SDS or safty data sheet
  more (RV-101, 102, 103) GHS.pdf
• Type of reagent
  Viral Expression
• Related techniques
  Viral Expression, Retroviral Expression, Retroviral Expression and Packaging, Platinum Retroviral Packaging Cell Lines
• Use of the reagent
  Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.
• Cellular use
  Higher retroviral yields: average titer 106 to 107 infectious units/mL with transient transfection
• Protocol information
  Longer stability: up to 4 months in the presence of drug selection
• Reagent properties
  Produces amphotropic retrovirus, which can infect human, mouse and rat cells
• Living modified organisme in this reagent or LMO
  Human cells transduced with retroviral genes
• Conjugation and form
  host specific
• Species compatible to
  human cells and cell lines and other cells
• Use of this test kit or product
  Molecular biology
• Antigen
  the sequence of the protein
• Buffer
  The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,40
• Molecular weight
  kiloDalton
• This reagent is purified
  0.98
• Description
  For cells, cell lines and tissues in culture till half confluency.

• Gene target
  Platinum-A   Retroviral   Packaging   Line   Amphotropic  
• Short name
  Platinum-A Retroviral Packaging Line, Amphotropic
• Alternative name
  Platinum-A Retroviral Packaging cellular Line, Amphotropic
• Tissue
  cell

MeSH Data

• Name
  Virus Cultivation
• Concept
  Scope note: Process of growing viruses in live animals, plants, or cultured cells.
• Tree numbers
  • E01.370.225.875.970
  • E05.200.875.970
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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