CPLA2 Antibody

• Catalog number
  R31157
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  CPLA2
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the CPLA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This CPLA2 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P47712
• Purity
  Antigen affinity
• Description
  Cytosolic Phospholipase A2, Group IVA is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).
• Immunogen
  An amino acid sequence from the C-terminus of human Phospholipase A2 (PNQAFKRLHDLMHFN) was used as the immunogen for this CPLA2 antibody.
• Storage
  After reconstitution, the CPLA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  CPLA2  
• Gene symbol
  PLA2G4C, PLA2G4A, PLA2G4B, KAT5
• Short name
  Anti-CPLA2
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to CPLA2
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9037
• Gene
  PLA2G4C
• Long gene name
  phospholipase A2 group IVC
• Synonyms gene name
  • phospholipase A2, group IVC (cytosolic, calcium-independent)
• Synonyms
  • cPLA2-gamma
• GenBank acession
  • AF065214
• Locus
  19q13.33
• Discovery year
  1998-11-20
• Entrez gene record
  8605
• Pubmed identfication
  • 9705332
• Classification
  • Phospholipases
• VEGA ID
  OTTHUMG00000183185

Gene info

• Identity
  HGNC:9035
• Gene
  PLA2G4A
• Long gene name
  phospholipase A2 group IVA
• Synonyms gene
  • PLA2G4
• Synonyms gene name
  • phospholipase A2, group IVA (cytosolic, calcium-dependent)
• Synonyms
  • cPLA2-alpha
• GenBank acession
  • M72393
• Locus
  1q31.1
• Discovery year
  1994-11-30
• Entrez gene record
  5321
• Pubmed identfication
  • 8175726
• RefSeq identity
  • NM_024420
• Classification
  • Phospholipases
  • C2 domain containing phospholipases
• VEGA ID
  OTTHUMG00000035512
• Locus Specific Databases
  • LRG_596

Gene info

• Identity
  HGNC:9036
• Gene
  PLA2G4B
• Long gene name
  phospholipase A2 group IVB
• Synonyms gene name
  • phospholipase A2, group IVB (cytosolic)
• Synonyms
  • cPLA2-beta
  • HsT16992
• GenBank acession
  • AF065215
• Locus
  15q15.1
• Discovery year
  1998-11-20
• Entrez gene record
  100137049
• Pubmed identfication
  • 9705332
• RefSeq identity
  • NM_001114633
• Classification
  • C2 domain containing phospholipases
  • Phospholipases
• VEGA ID
  OTTHUMG00000156809

Gene info

• Identity
  HGNC:5275
• Gene
  KAT5
• Long gene name
  lysine acetyltransferase 5
• Synonyms gene
  • HTATIP
• Synonyms gene name
  • HIV-1 Tat interactive protein, 60kDa
  • K(lysine) acetyltransferase 5
• Synonyms
  • TIP60
  • PLIP
  • cPLA2
  • HTATIP1
  • ESA1
  • ZC2HC5
• Synonyms name
  • Tat interacting protein, 60kDa
  • K-acetyltransferase 5
• GenBank acession
  • U74667
• Locus
  11q13.1
• Discovery year
  2000-04-13
• Entrez gene record
  10524
• Pubmed identfication
  • 8607265
• RefSeq identity
  • NM_006388
• Classification
  • Zinc fingers C2HC-type
  • MYST type domain containing lysine acetyltransferases
• VEGA ID
  OTTHUMG00000166617

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products