PKN1 Antibody (C-Terminal)
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Catalog number
F53997-0.2ML
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Price
Please ask
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Size
0.2 ml
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Category
Antibody
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Concentration
Antibody in 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
PKN1 (C-Terminal)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 1:2000
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Notes
The stated application concentrations are suggested starting points. Titration of the PKN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This PKN1 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
Q16512
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Purity
Antigen affinity
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Description
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.
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Immunogen
Amino acids 836-870 from the human protein were used as the immunogen for this PKN1 antibody.
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Storage
Aliquot the PKN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
PKN1
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Short name
Anti-PKN1 (C-Terminal)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to PKN1 (C-Terminal)
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Alternative technique
antibodies
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Alternative to gene target
protein kinase N1, DBK and PAK-1 and PAK1 and PKN and PKN-ALPHA and PRK1 and PRKCL1, PKN1 and IDBG-33268 and ENSG00000123143 and 5585, transferase activity, nuclei, Pkn1 and IDBG-172377 and ENSMUSG00000057672 and 320795, PKN1 and IDBG-642677 and ENSBTAG00000017037 and 509080
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Gene info
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Identity
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Gene
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Long gene name
protein kinase N1
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Synonyms gene
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1997-10-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2 domain containing protein kinases
- AGC family kinases
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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