-
Synonyms
PKC, PKC, Protein kinase C zeta
-
Alternative_names
PKC, PKC, Protein kinase C zeta
-
Description
Phosphorylate a wide variety of protein targets
-
Recombinant
Yes
-
Source
Baculovirus (Sf9 insect cells)
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Activity Specifications test method
93 nmol phosphate incorporated into CREBtide per minute per mg protein at 300C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
-
Molecular Weight
93.0 kDa
-
Storage Temp
-80°C
-
Shipping
dry ice
-
Shelf Life
12 months
-
Concentration
5µg/50 µl
-
Appearance
Liquid
-
Physical form description
Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30% glycerol).
-
Background Information
PKCζ (PKC zeta) is an atypical isoform of the PKC family. PKCζ is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Treatment of cells with phorbol ester which activates PKCα, γ, δ, and ε isoforms in NIH3T3 cells significantly reduced proliferation of cells. Overexpression of PKCζ and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCζ also potentiated phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. The effects of PKCζ overexpression on proliferation and MAP kinase activation are proportional to the levels of PKCζ expression. PKCζ as an upstream modulator of p70S6K, an important regulator of cell proliferation (2). Kinase-inactive PKCζ mutant antagonized activation of p70S6K by epidermal growth factor, PDK-1, and activated Cdc42 and PI3-K. Overexpression of a constitutively active PKCζ mutant (myristoylated PKCζ [myr-PKCzeta]) only modestly activated p70S6K but this mutant cooperated with PDK-1 for the activation of p70S6K. PDK-1-induced activation of a C-terminal truncation mutant of p70S6K was also enhanced by myr-PKCζ. p70S6K can associate with both PDK-1 and PKCz in vivo in a growth factor-independent manner, while PDK-1 and PKCζ can also associate with each other, suggesting the existence of a multimeric PI3-K signalling complex.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
