PKC iota, Active
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Catalog number7705-5
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PricePlease ask
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Size5 ug
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SynonymsPKC, Protein kinase C iota
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Alternative_namesPKC, Protein kinase C iota
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DescriptionPhosphorylate a wide variety of protein targets
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RecombinantYes
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SourceBaculovirus (Sf9 insect cells)
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test method664 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay in 25µl reaction volume).
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Molecular Weight~98.0 kDa
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Storage Temp-80°C
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ShippingDry ice
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Shelf Life12 months
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Concentration5µg/50 µl
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AppearanceLiquid
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Physical form descriptionRecombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
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Background InformationPKC ι is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKC ι showed greatest homology to PKC zeta, with 72% identity overall rising to 84% in the catalytic domain. In contrast, the homology of PKC ι to the other isoforms is less pronounced, with < 53% identity even in the highly conserved catalytic region. PKC ι transcript is present predominantly in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. PKC ι is stimulated by tumor necrosis factor α (TNF-α) and is required for the activation of NF-kB by this cytokine. Cell transfections with a PKC ι dominant negative mutant abolished TNF-α-induced NFkB-dependent transcription. PKC ι can modify vulnerability of neural cells to apoptosis induced by amyloid β-peptide (ABP), a cytotoxic peptide linked to neuronal degeneration in Alzheimer's disease. Associated with the increased resistance to apoptosis are improved mitochondrial function and reduced activity of caspases. In addition, ABP-induced increases in levels of oxidative stress and intracellular calcium levels were attenuated in cells overexpressing PKC ι.
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Gene target
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Gene symbolPRKCH, PRKD1, PRRT2, PRKDC
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Short namePKC iota, Active
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Alternative namePKC iota, functionnal
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Gene info
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Identity
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Gene
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Long gene nameprotein kinase C eta
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Synonyms gene
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Synonyms gene name
- protein kinase C, eta
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-06-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2 domain containing protein kinases
- AGC family kinases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein kinase D1
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Synonyms gene
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Synonyms gene name
- protein kinase C, mu
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Synonyms
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Locus
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Discovery year1994-05-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pleckstrin homology domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproline rich transmembrane protein 2
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Synonyms gene
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Synonyms gene name
- infantile convulsions and paroxysmal choreoathetosis
- dystonia 10
- proline-rich transmembrane protein 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2005-11-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- interferon induced transmembrane protein domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases