PKC delta, Active

• Catalog number
  7739-5
• Price
  Please ask
• Size
  5 ug

• Synonyms
  PKC, Protein kinase C delta
• Alternative_names
  PKC, Protein kinase C delta
• Description
  Phosphorylate a wide variety of protein targets
• Recombinant
  Yes
• Source
  Baculovirus (Sf9 insect cells)
• Purity by SDS PAGE
  ≥80%
• Assay
  SDS-PAGE
• Activity Specifications test method
  302 nmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
• Molecular Weight
  104.0 kDa
• Storage Temp
  -80°C
• Shipping
  dry ice
• Shelf Life
  12 months
• Concentration
  5µg/50 µl
• Appearance
  Liquid
• Physical form description
  Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Background Information
  Protein kinase C delta (PKC delta) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It is a 79 kd protein kinase that shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+ (1). Phosphatidylinositol is the most potent activator of PKC delta. Apparent kinetic constants for synthetic oligopeptides (MBP4-14, EGFR peptide and epsilon-peptide) are quite different between PKC delta and other PKCs. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells (2). PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters. PKC delta is partially associated with the insoluble fraction in cells even in the absence of phorbol 12-myristate 13-acetate (PMA). Upon PMA stimulation, both it translocate to the insoluble fraction of cell homogenates (3). Overexpression of PKC-delta induces significant changes in morphology and causes the cells to grow more slowly and to a decreased cell density in confluent cultures. These changes are accentuated by treatment with PMA. None of the PKC-delta overexpressers grow in soft agar with or without PMA.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet

• Gene target
  PKC   delta   Active  
• Gene symbol
  PRKCH, PRKD1, PRRT2, PRKDC
• Short name
  PKC delta, Active
• Alternative name
  PKC delta, functionnal

Gene info

• Identity
  HGNC:9403
• Gene
  PRKCH
• Long gene name
  protein kinase C eta
• Synonyms gene
  • PRKCL
• Synonyms gene name
  • protein kinase C, eta
• Synonyms
  • PKC-L
  • PKCL
• GenBank acession
  • M55284
• Locus
  14q23.1
• Discovery year
  1992-06-26
• Entrez gene record
  5583
• Pubmed identfication
  • 1986216
  • 1545821
• RefSeq identity
  • NM_006255
• Classification
  • C2 domain containing protein kinases
  • AGC family kinases
• VEGA ID
  OTTHUMG00000152341

Gene info

• Identity
  HGNC:9407
• Gene
  PRKD1
• Long gene name
  protein kinase D1
• Synonyms gene
  • PRKCM
• Synonyms gene name
  • protein kinase C, mu
• Synonyms
  • PKCM
  • PKD
  • PKC-mu
• Locus
  14q12
• Discovery year
  1994-05-16
• Entrez gene record
  5587
• Pubmed identfication
  • 8119958
  • 10965134
• RefSeq identity
  • NM_002742
• Classification
  • Pleckstrin homology domain containing
• VEGA ID
  OTTHUMG00000140203

Gene info

• Identity
  HGNC:30500
• Gene
  PRRT2
• Long gene name
  proline rich transmembrane protein 2
• Synonyms gene
  • ICCA
  • DYT10
• Synonyms gene name
  • infantile convulsions and paroxysmal choreoathetosis
  • dystonia 10
  • proline-rich transmembrane protein 2
• Synonyms
  • FLJ25513
  • DKFZp547J199
  • IFITMD1
  • FICCA
  • DSPB3
  • PKC
  • EKD1
• Synonyms name
  • interferon induced transmembrane protein domain containing 1
  • dispanin subfamily B member 3
• GenBank acession
  • BC011405
• Locus
  16p11.2
• Discovery year
  2005-11-25
• Entrez gene record
  112476
• Pubmed identfication
  • 22101681
  • 22243967
  • 29346777
• RefSeq identity
  • NM_145239
• Classification
  • interferon induced transmembrane protein domain containing
• VEGA ID
  OTTHUMG00000177142

Gene info

• Identity
  HGNC:9413
• Gene
  PRKDC
• Long gene name
  protein kinase, DNA-activated, catalytic subunit
• Synonyms gene
  • HYRC
  • HYRC1
• Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
• Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
• Synonyms name
  • DNA-dependent protein kinase catalytic subunit
• Locus
  8q11.21
• Discovery year
  1993-11-09
• Entrez gene record
  5591
• Pubmed identfication
  • 7638222
• RefSeq identity
  • NM_001081640
• Classification
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000164239
• Locus Specific Databases
  • LRG_162

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