Guinea Pig IgG (Fab) (FITC)
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Catalog number
MBS538954
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Price
Please ask
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Size
1 mg
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Products_type
Immunoglobulin
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Products_short_name
[Guinea Pig IgG (Fab) (FITC)]
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Products_name_syn
[Guinea Pig Immunoglobulin G (Fab) (FITC)]
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Products_gene_name
[IgG]
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Purity
Guinea Pig IgG (Fab) (FITC) was purified by delipidation, salt fractionation, ion exchange chromatography followed by dialysis.
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Form
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml BSA, and 0.01% NaN3. Immunoglobulin and protease free.
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Storage_stability
Store at 4 degree C until reconstitution. Following reconstitution aliquot and freeze at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
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Tested_application
Immunofluorescence (IF)
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Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Description
Guinea pig ELISA kits for plasma and sera samples are used to study human genes through the guinea pig model (Cavia porcellus), also called the cavy rodent model. After mouzes and rats Guinea pigs are easy in maintained laboratory animals. cDNAs of Guinea pigs are also very popular.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans.
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Short name
IgG (Fab) (FITC)
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Technique
FITC, Fab, IgG, fabs, Fluorescein, IgGs
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Host
Guinea Pig
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Isotype
IgG, Fab fragment, IgG
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Label
FITC
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Species
Guinea Pig, Guinea Pigs, Pigs and Guinea pigs
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Alternative name
Guinea swine Immunoglobulin G (Fab) (fluorecein)
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Alternative technique
fluorescine, seconairy, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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