PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit
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Catalog number
K314-500
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Price
Please ask
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Size
500 assays
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Description
Ultra-sensitive Assay, HTS
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Summary
• Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications- Measurement of released LDH in culture medium • Evaluation of cytotoxicity effect of test compounds
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Detection Method
Fluorescence (Ex/Em = 535/587 nm)
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Applications
• Measurement of released LDH in culture medium • Evaluation of cytotoxicity effect of test compounds
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Sample Type
Adherent or Suspension Cells
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Features Benefits
• Highly-Sensitive: Detects as less as 100 cells. • Rapid protocol: requires less than 20 min. • High-throughput adaptable.
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. BioVision’s PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.
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Test
Biovision supplies other types of Assays as 1.
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Conjugate
Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit
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Gene target
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Short name
™ LDH-Cytotoxicity Fluorometric Assay Kit
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Technique
Assay, assays
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Host
Assay
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Alternative name
PicoProbe™ LDH-Cytotoxicity fluorescence test reagent
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Alternative technique
arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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