PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit

  • Catalog number
    K314-500
  • Price
    Please ask
  • Size
    500 assays
  • Description
    Ultra-sensitive Assay, HTS
  • Summary
    • Detection method- Fluorescence (Ex/Em = 535/587 nm) • Applications- Measurement of released LDH in culture medium • Evaluation of cytotoxicity effect of test compounds
  • Detection Method
    Fluorescence (Ex/Em = 535/587 nm)
  • Applications
    • Measurement of released LDH in culture medium • Evaluation of cytotoxicity effect of test compounds
  • Sample Type
    Adherent or Suspension Cells
  • Features Benefits
    • Highly-Sensitive: Detects as less as 100 cells. • Rapid protocol: requires less than 20 min. • High-throughput adaptable.
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. BioVision’s PicoProbe™ LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.
  • Test
    Biovision supplies other types of Assays as 1.
  • Conjugate
    Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit
  • Gene target
  • Short name
    ™ LDH-Cytotoxicity Fluorometric Assay Kit
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    PicoProbe™ LDH-Cytotoxicity fluorescence test reagent
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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