PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric)

• Catalog number
  K248-100
• Price
  Please ask
• Size
  100 assasys

• Description
  Rapid, sensitive & simple assay to measures D-2Hydroxyglutarate Dehydrogenase activity
• Summary
  • Detection method- Fluorescence (Ex/Em=535/587 nm) • Application- Detection of D-2-Hydroxyglutarate Dehydrogenase activity
• Detection Method
  Fluorescence (Ex/Em=535/587 nm)
• Species Reactivity
  Mammalian
• Applications
  Detection of D-2-Hydroxyglutarate Dehydrogenase activity
• Sample Type
  • Purified recombinant protein • Cell and tissue lysate
• Features Benefits
  • Rapid, sensitive and convenient • The kit measures total Activity of D-2-Hydroxyglutatare Dehydrogenase Activity with limit of quantification 4 µU/µl using recombinant D2HGDH
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  D-2-Hydroxyglutarate Dehydrogenase (D2HGDH: EC 1.1.99.2) is a mitochondrial enzyme which catalyzes the conversion of D-2-Hydroxyglutarate into 2-Oxoglutarate. In humans, mutation of D2HGDH gene could reduce D2HGDH activity which will cause the D-2-Hydroxyglutarate accumulation in body fluids. Increased D-2-Hydroxyglutarate level in body fluids leads to D-2-Hydroxyglutaric Aciduria, a neurometabolic disorder characterized as developmental delay, epilepsy, hypotonia, and dysmorphic features. BioVision’s PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay kit provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples. In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces PicoProbe™ to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µl of D2HGDH activity.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Conjugate
  Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit

• Gene target
  D-2-Hydroxyglutarate   Dehydrogenase   Kit   Fluorometric  
• Gene symbol
  RAB3D, NCAPD2, HMGN2P46, DDT, SNAR-D, PDPN, DGCR11, IGHD2-15
• Short name
  ™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:9779
• Gene
  RAB3D
• Long gene name
  RAB3D, member RAS oncogene family
• Synonyms gene
  • GOV
• Synonyms
  • RAB16
  • D2-2
  • RAD3D
• Synonyms name
  • Rab3D upregulated with myeloid differentiation
  • glioblastoma overexpressed
• GenBank acession
  • AF081353
• Locus
  19p13.2
• Discovery year
  1999-06-02
• Entrez gene record
  9545
• Pubmed identfication
  • 10023084
  • 10072586
• RefSeq identity
  • NM_004283
• Classification
  • RAB, member RAS oncogene GTPases
• VEGA ID
  OTTHUMG00000180835

Gene info

• Identity
  HGNC:24305
• Gene
  NCAPD2
• Long gene name
  non-SMC condensin I complex subunit D2
• Synonyms gene name
  • non-SMC condensin I complex, subunit D2
• Synonyms
  • CNAP1
  • hCAP-D2
  • CAP-D2
  • KIAA0159
• Synonyms name
  • chromosome condensation related SMC associated protein 1
• GenBank acession
  • D63880
• Locus
  12p13.31
• Discovery year
  2006-09-04
• Entrez gene record
  9918
• Pubmed identfication
  • 8590280
  • 10958694
• RefSeq identity
  • NM_014865
• Classification
  • Condensin I subunits
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000168513

Gene info

• Identity
  HGNC:26817
• Gene
  HMGN2P46
• Long gene name
  high mobility group nucleosomal binding domain 2 pseudogene 46
• Synonyms gene
  • C15orf21
• Synonyms gene name
  • chromosome 15 open reading frame 21
  • high-mobility group nucleosomal binding domain 2 pseudogene 46
• Synonyms
  • FLJ39426
  • D-PCa-2
• GenBank acession
  • AK096745
• Locus
  15q21.1
• Discovery year
  2004-02-23
• Entrez gene record
  283651
• Pubmed identfication
  • 15027122
  • 12727900
  • 24043589
• RefSeq identity
  • NR_022014

Gene info

• Identity
  HGNC:2732
• Gene
  DDT
• Long gene name
  D-dopachrome tautomerase
• Synonyms
  • DDCT
  • MIF2
  • MIF-2
  • D-DT
• Synonyms name
  • D-dopachrome decarboxylase
• GenBank acession
  • U49785
• Locus
  22q11.23
• Discovery year
  1998-03-06
• Entrez gene record
  1652
• Pubmed identfication
  • 9480844
  • 9729470
  • 22507380
  • 29184004
• RefSeq identity
  • NM_001355
• VEGA ID
  OTTHUMG00000150798

Gene info

• Identity
  HGNC:34324
• Gene
  SNAR-D
• Long gene name
  small NF90 (ILF3) associated RNA D
• Synonyms gene name
  • small ILF3/NF90-associated RNA D
• GenBank acession
  • EU071071
• Locus
  19q13.33
• Discovery year
  2008-07-03
• Entrez gene record
  100170227
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_024243
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:29602
• Gene
  PDPN
• Long gene name
  podoplanin
• Synonyms
  • T1A-2
  • Gp38
  • aggrus
  • GP40
  • PA2.26
  • D2-40
• Synonyms name
  • lung type I cell membrane associated glycoprotein
• GenBank acession
  • AB127958
  • AY194238
• Locus
  1p36.21
• Discovery year
  2005-07-08
• Entrez gene record
  10630
• Pubmed identfication
  • 10393083
  • 9651190
• RefSeq identity
  • NM_006474
• VEGA ID
  OTTHUMG00000007912

Gene info

• Identity
  HGNC:17226
• Gene
  DGCR11
• Long gene name
  DiGeorge syndrome critical region gene 11
• Synonyms gene name
  • DiGeorge syndrome critical region gene 11
  • DiGeorge syndrome critical region gene 11 (non-protein coding)
• Synonyms
  • DGS-D
• GenBank acession
  • L77561
• Locus
  22q11.21
• Discovery year
  2002-11-13
• Entrez gene record
  25786
• Pubmed identfication
  • 8776594
• Classification
  • Long non-coding RNAs with non-systematic symbols
• VEGA ID
  OTTHUMG00000186100

Gene info

• Identity
  HGNC:5489
• Gene
  IGHD2-15
• Long gene name
  immunoglobulin heavy diversity 2-15
• Synonyms
  • IGHD215
  • D2
• GenBank acession
  • J00234
• Locus
  14q32.33
• Discovery year
  2000-04-04
• Entrez gene record
  28503
• Pubmed identfication
  • 7312051
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152430

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