Phosphoglycerate mutase 1, human recombinant
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Catalog numberP1105-50
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PricePlease ask
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Size50 µg
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SynonymsPhosphoglycerate mutase isozyme B, PGAM-B, PGAMA
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Alternative_namesPhosphoglycerate mutase isozyme B, PGAM-B, PGAMA
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DescriptionCatalyzes the reversible reaction of 3-PGA to 2-PGA in the glycolytic pathway.
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥90%
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AssaySDS-PAGE
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Activity Specifications test methodMeasured by its ability convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
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Biological activitySpecific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
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Results>300 units/mg
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Unit DefinitionOne unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.
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Molecular Weight30.9 kDa
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Storage Temp-20°C
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ShippingGel pack
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Shelf Life12 months
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Concentration1 mg/ml
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AppearanceLiquid
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Physical form descriptionIn 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT
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Background InformationPGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3- phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fastmigrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Amino acid sequenceMGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolPGAM1, PGAM1P2, PGAM1P3
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Short namePhosphoglycerate mutase 1, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namePhosphoglycerate mutase 1, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namephosphoglycerate mutase 1
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Synonyms gene
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Synonyms gene name
- phosphoglycerate mutase 1 (brain)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Phosphoglycerate mutases
- Bisphosphoglycerate phosphatases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphoglycerate mutase 1 pseudogene 2
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Locus
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Discovery year2011-07-28
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephosphoglycerate mutase 1 pseudogene 3
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Locus
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Discovery year2011-07-28
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data