Phospho-ERK1/2 (Thr202/Tyr204) Translocation Assay Kit (Cell-Based)

  • Catalog number
    K696-50
  • Price
    Please ask
  • Size
    50 assays
  • Description
    A cell-based assay kit that detect the nuclear translocation of activated ERK1/2 in fixed mammalian cells.
  • Summary
    • Detection method- fluorescence microscope capable of measuring EX at 570 nm and equipped with UV filter for DAPI • Species reactivity- Human, mouse, rat • Application- Detection of nuclear translocation of phosphorylated-ERK1/2 in mammalian cells
  • Detection Method
    fluorescence microscope capable of measuring EX at 570 nm and equipped with UV filter for DAPI
  • Species Reactivity
    Human, mouse, rat
  • Applications
    Detection of nuclear translocation of phospo-ERK1 and phospo-ERK2 in mammalian cells
  • Sample Type
    Adherent or suspension mammalian cell cultures
  • Features Benefits
    • Detection of subcellular localization of activated ERK1/2 in mammalian cells • Screening and characterizing effectors of ERK1/2 kinases • Studying of cell signaling, cell division and cell proliferation mechanism
  • Storage Conditions
    -20°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Mitogen-activated protein kinases (MAPKs) are proline-directed serine and threonine protein kinases that regulate numerous physiological cell responses including: embryogenesis, cell differentiation, proliferation, migration, apoptosis and death. Extracellular signal-regulated kinases (ERKs) 1 and 2 (ERK1/2), also known as p44 MAPK and p42 MAPK respectively, belong to one of the five major groups of MAPKs. Closely-related ERK1/2 isoforms are uniquely activated by several extracellular signals including growth factors, cytokines, hormones, and neuro-transmitters. Activation of ERK1/2 by the upstream kinases MEK1 and MEK2 occurs via dual phosphorylation on specific threonine (Thr202) and tyrosine (Tyr204) residues on the T*EY* motif. MEK1 and MEK2 are activated through receptors (tyrosine kinases or integrins) via pathways involving adaptor proteins, guanine nucleotide exchange factors, small GTP binding proteins, and MAPKKs. Activated ERK1/2 phosphorylates both, cytosolic (SOS, MNK1/2, RSKs) and nuclear targets. In the nucleus, it affects gene expression and DNA replication by the phosphorylation of MSK 1 and 2 and the transcription factors Elk-1, Sap1, and Sap2. In cultured cells, growth factors or mitogens induce rapid and transient translocation of activated ERK1/2 to nucleus. Different cell lines exhibit various duration, magnitude, and subcellular localization of activated/phosphorylated ERK1/2. The response of the protein may differ even within the same cell line depending on the dose and cell density. BioVision’s Phospho-ERK1/2 (Thr202/Tyr204) Translocation Assay Kit provides a simple and complete assay in a ready-to-use format to visualize the translocation of activated ERK1/2 between cytoplasmic and nuclear compartments in mammalian cells.
  • Test
    Biovision supplies other types of Assays as 1.
  • Additional description
    For cells, cell lines and tissues in culture till half confluency.
  • Gene target
  • Gene symbol
    MAPK3
  • Short name
    Phospho-ERK1/2 (Thr202/Tyr204) Translocation Assay Kit ( -Based)
  • Technique
    Assay, assays
  • Host
    Assay
  • Alternative name
    phosphorilated-ERK1/2 (Thr202/Tyr204) Translocation test reagent (cellular-Based)
  • Alternative technique
    arrays, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    cell
Gene info
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee