CDC25A Antibody (phospho-S124)
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Catalog number
F48374-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
CDC25A (phospho-S124)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC, ELISA
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Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
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Notes
Titration of the phospho-CDC25A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This phospho-CDC25A antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P30304
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Purity
Antigen affinity
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Description
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
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Immunogen
This phospho-CDC25A antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS124 of human CDC25A.
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Storage
Aliquot the phospho-CDC25A antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
CDC25A
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Short name
Anti-CDC25A (phospho-S124)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to CDC25A (phospho-S124)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
cell division cycle 25A
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1992-07-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Class III Cys-based CDC25 phosphatases
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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