Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) w/ 15mM HEPES buffer and Trace elements w/o L-Glutamine, Phenol red and Sodium bicarbonate

  • Catalog number
    AT192A-1LX18
  • Price
    Please ask
  • Size
    18X1L
  • Description
    Eagle Medium Blend / Dulbecco Nutrient F-12 ham (DMEM / F12, 1: 1 blend) was originally formulated for rat neuroblastoma cells and MDCK cells. prevents the initial increase in pH that tends to occur at the beginning of a culture and increases the buffering capacity of the medium.
  • Shelf Life
    Use before the expiry date
  • Storage conditions
    stored at 2-8°C
  • Appearance
    Off-white to Creamish white, homogenous powder
  • Solubility
    Clear solution at 15.3 gms/L.
  • Use
    used in laboratory chemicals, Manufacture of substances
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia. There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Group
    media
  • Conjugation
    red dye
  • Gene target
  • Gene symbol
    F12, HLA-F, SNAR-F, H3C11, IGHV1-69-2, KLRC4, PIRC55, PIRC54, PIRC53
  • Short name
    Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) w/ 15mM Trace elements w/o L-Glutamine, Phenol red Sodium bicarbonate
  • Technique
    Medium, cell culture Media
  • Label
    Red
  • Alternative name
    Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 Ham (DMEM/ F12, 1:1 Mixture) w/ 15mM HEPES solution and Trace elements w/o L-Glutamine, Phenol red and Sodium bicarbonate
  • Alternative technique
    cellculture
  • Virus
    hhv
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    small NF90 (ILF3) associated RNA F
  • Synonyms gene name
    • small nuclear ILF3/NF90-associated RNA F
    • small ILF3/NF90-associated RNA F
  • GenBank acession
  • Locus
  • Discovery year
    2008-06-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    killer cell lectin like receptor C4
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-09-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Killer cell lectin like receptors
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 55
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 54
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
  • Gene
  • Long gene name
    piwi-interacting RNA cluster 53
  • Locus
    12
  • Discovery year
    2009-11-05
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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