Perchlorate Free Assay Kit

Perchlorate Free Assay Kit is available 2 times from Biovision labs

K226-100 | Perchlorate-Free Hydroxyproline Assay Kitsize: 100 assays | 679.07 USD

  • Catalog number
    K226-100
  • Supplier
  • Price
    679.07 USD
  • Size
    100 assays
  • Description
    Hydroxyproline ((2S,4R)-4-hydroxyproline, Hyp) is a nonproteinogenic amino acid formed by the enzyme prolyl-4-hydroxylase, which catalizes the post-translational modification of polypeptide proline residues following protein synthesis. In animals, hydroxyproline is found almost entirely in collagen, which contains approximately 12-14% hydroxyproline by mass (the fibrilous protein elastin also contains small quantities of hydroxyproline). Hydroxyproline in tissue hydrolysates is thus a direct measure of the amount of collagen present. Collagen is the most abundant protein in mammals and many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. The classical assay for determination of Hyp is based upon oxidation of Hyp to a pyrrole intermediate, followed by reaction with Ehrlich’s reagent dissolved in concentrated perchloric acid (HClO4). Perchloric acid is a hazardous material that is both toxic and highly reactive, requiring special handling and waste-disposal protocols. BioVision’s Hydroxyproline Assay Kit II (Perchlorate-Free) uses a proprietary acidic developer solution to accurately measure Hyp in tissue and protein/peptidase hydrolysates without the use of hazardous perchlorates. It is a quick and convenient protocol, yielding a brightly-colored chromophore with an absorbance peak at 560 nm. The assay can detect a minimum of 0.05 µg hydroxyproline per well in a 96-well format and generates colorimetric results that are virtually identical to the classical perchloric acid-based method.
  • Highlights
    Convenient and easy to use kit with vert simple procedure because it takes less than ~60 minutes. Faster than Perchlorate-Based Assay, PCA-free
  • Kit reactivity
    Mammalian
  • Kit is besf for
    Estimation of hydroxyproline concentration in various biological samples
  • Other name
    Hydroxyproline Assay Kit
  • Detection procedure
    Absorbance (OD = 560 nm).
  • Kit contents
    The Perchlorate-Free Hydroxyproline Assay Kit contains Oxidation Buffer, Chloramine T Concentrate, Developer Solution, DMAB Concentrate (in DMSO), Hydroxyproline Standard (1 mg/ml) and a Microplate Sealing Film
  • Samples
    Animal tissues protein/peptide hydrolysates serum urine
  • Assay type
    Hydroxyproline
  • Test
    Biovision supplies other types of Assays as 1.
MeSH Data
  • Name
  • Concept
    Registry number:RMB44WO89X
    Scope note:A hydroxylated form of the imino acid proline. A deficiency in ASCORBIC ACID can result in impaired hydroxyproline formation.
    CASN1 number:L-Proline, 4-hydroxy-, trans-
  • Tree numbers
    • D12.125.072.401.623.478
Additional information
Product images

K406-100 | Perchlorate-Free Total Collagen Assay Kitsize: 100 assays | 679.07 USD

Ajax processing
  • Storage
    Store this kit at -20°C for 12 months
  • Delivery
    This product is shipped with gel pack
  • Datasheet
    Please contact us to inquire the datasheet and an example certificate of analysis
  • Released
    This assay kit is on the market since May 2017
  • Technique
    Assay
  • Host
    Assay
  • Alternative technique
    arrays
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
  • Tissue
    total
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