Total Collagen Assay Kit (Perchlorate-Free)

• Catalog number
  K406-100
• Price
  Please ask
• Size
  100 Reactions

• Description
  A simple, sensitive & easy colorimetric assay to quantitate total collagen levels.
• Summary
  • Detection method- Absorbance (OD = 560 nm). • Sample Types- Mammalian tissues, protein/peptide hydrolysates, serum, Urine • Applications- Measurement of collagen in various sample types.
• Detection Method
  Detection method- Absorbance (OD = 560 nm).
• Species Reactivity
  Mammalian tissues, protein/peptide hydrolysates, serum, urine
• Applications
  Measurement of collagen in various sample types.
• Sample Type
  • Mammalian tissues • Protein/peptide hydrolysates • Serum • Urine
• Features Benefits
  • Simple, fast and convenient assay • Can detect as low as 0.5 µg of collagen in a variety of samples • Simple procedure; takes less than ~60 minutes • Faster than Perchlorate-Based Assay • PCA-free
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Collagen is the most abundant protein in mammals, accounting for nearly 30% of body protein content. Collagen is an extracellular scaffolding protein found in skin, tendons, bone, cartilage, ligaments and muscles, amongst other tissues. There are more than twenty different characterized types of collagen, but the fibrilous types I, II and III comprise more than 90% of the total collagen in mammals. Collagen has a triple helical structure, consisting of three ‘preprocollagen’ strands with a repeating Gly-X-Hyp tripeptide sequence, where X is any amino acid and Hyp is hydroxyproline, a post-translationally modified amino acid formed from proline by the enzyme prolyl-4-hydroxylase. In animals, hydroxyproline is found almost entirely in collagen (which contains approximately 12-14% hydroxyproline by mass) and contributes to its structural rigidity and high tensile strength. Hydroxyproline is thus a direct measure of the amount of collagen present in tissues. Many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. BioVision’s Total Collagen Assay Kit II (Perchlorate-Free) is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples. The assay is based upon hydrolysis of samples to yield free Hyp, which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm. The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, obviating the need for any special handling and waste-disposal protocols. The assay can detect a minimum of 0.5 µg collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical perchloric acid-based method
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Gene
  Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )

• Gene target
  Collagen   Kit   Perchlorate-Free  
• Short name
  Collagen Assay Kit (Perchlorate-Free)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Total Collagen test reagent (Perchlorate-Free)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

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