Human CellExp™ PDGF-BB, human recombinant

• Catalog number
  7276-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2
• Description
  Mitogenic during early developmental stages
• Recombinant
  1
• Source
  HEK 293 cells
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
• Biological activity
  The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml
• Molecular Weight
  This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   PDGF-BB  
• Gene symbol
  PDGFA
• Short name
  CellExp™ PDGF-BB, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ PDGF-BB, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8799
• Gene
  PDGFA
• Long gene name
  platelet derived growth factor subunit A
• Synonyms gene name
  • platelet-derived growth factor alpha polypeptide
• Synonyms
  • PDGF1
  • PDGF-A
• Synonyms name
  • PDGF A-chain
  • platelet-derived growth factor alpha chain
• Locus
  7p22.3
• Discovery year
  1986-01-01
• Entrez gene record
  5154
• Pubmed identfication
  • 1505216
  • 2536956
• VEGA ID
  OTTHUMG00000151412

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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