PCR Quick Screening Kit, InsertFinder™

• Catalog number
  K903-500
• Price
  Please ask
• Size
  500 samples

• Description
  Directly screen colonies from plate
• Summary
  • Application- Kit is designed to quickly screen large number of cloning inserts
• Sample Type
  Bacteria and yeast
• Features Benefits
  • Procedure is quick and simple • Clones can be analyzed directly on an agarose gel
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Identification of clones that contain the desired DNA insert is an essential step in molecular cloning procedure. Traditional methods for identifying the correct DNA inserts require growing up individual colonies, isolating plasmid DNAs, and then digesting with restriction enzymes to analyze each individual clone. Such traditional procedures are very time consuming, laborious, and expensive. The new InsertFinderTM PCR Quick Screening Kit is designed to quickly screen clone candidates using PCR technology. The procedure allows screen of colonies directly from plate, without growing up the cultures. The proprietary Cell Lysis Buffer provided with the kit can efficiently lyse cells (bacteria or yeast) without interfering with PCR reactions. The PCR Enhancer included in the kit allows amplification of hairpin structure or GC-rich region efficiently. PCR products can directly be analyzed on an agarose gel without enzyme digestions. The kit provides a simple, convenient, and economical way to identify correct inserts. It is also suitable for screening large numbers of colonies.
• Data Sheet Link
• Group
  PCR, polymerase chain reaction
• About
  TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
• Properties
  Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.

• Gene target
  Quick   Screening   Kit   InsertFinder™  
• Short name
  PCR Quick Screening Kit, InsertFinder™
• Technique
  PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
• Alternative name
  PCR test kit Quick detection reagent, InsertFinder™
• Alternative technique
  kits, dna-amplification
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

MeSH Data

• Name
  Nucleic Acid Amplification Techniques
• Concept
  Scope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
• Tree numbers
  • E05.393.620
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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