PCAF bromodomain (492-658 aa), human recombinant
-
Catalog number
1137-500
-
Price
Please ask
-
Size
500 ug
-
-
Synonyms
p300/CBP-associated factor; KAT2B
-
Alternative_names
p300/CBP-associated factor; KAT2B
-
Description
An enzyme that acetylates histones
-
Recombinant
Yes
-
Source
E. coli
-
Purity by SDS PAGE
≥95%
-
Assay
SDS-PAGE
-
Activity Specifications test method
≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)
-
Biological activity
≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)
-
Results
≥0.2 U/mg
-
Unit Definition
One unit of HAT activity is the amount of enzyme that generates 1.0 µmol of CoA per minute at 30°C under kit assay conditions.
-
Storage Temp
-20°C
-
Shipping
gel pack
-
Shelf Life
12 months
-
Appearance
Liquid
-
Physical form description
30% Glycerol in PBS
-
Background Information
The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. P300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
KAT2A, MIR658, MIR492, KAT2B
-
Short name
PCAF bromodomain (492-658 aa), recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Human, Humans
-
Alternative name
PCAF bromodomain (492-658 aa), H. sapiens Rec.
-
Alternative technique
rec
-
Gene info
Gene info
-
Identity
-
Gene
-
Long gene name
microRNA 658
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year
2006-06-12
-
Entrez gene record
-
RefSeq identity
-
Classification
Gene info
-
Identity
-
Gene
-
Long gene name
microRNA 492
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year
2005-07-15
-
Entrez gene record
-
RefSeq identity
-
Classification
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products