PCAF bromodomain (492-658 aa), human recombinant

  • Catalog number
    1137-500
  • Price
    Please ask
  • Size
    500 ug
  • Synonyms
    p300/CBP-associated factor; KAT2B
  • Alternative_names
    p300/CBP-associated factor; KAT2B
  • Description
    An enzyme that acetylates histones
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    ≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)
  • Biological activity
    ≥0.2 U/mg as determined by the HAT (H4) Activity Fluorometric Assay Kit (Cat # K336-100)
  • Results
    ≥0.2 U/mg
  • Unit Definition
    One unit of HAT activity is the amount of enzyme that generates 1.0 µmol of CoA per minute at 30°C under kit assay conditions.
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Appearance
    Liquid
  • Physical form description
    30% Glycerol in PBS
  • Background Information
    The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. P300/CBP-associated factor (PCAF) is a transcriptional coactivator that works both as a histone lysine acetyltransferase, through its HAT domain, and as an acetyl-lysine reader through its conserved bromodomain located directly C-terminal to the HAT domain. The PCAF bromodomain binds acetylated histone H3 and H4 as well as non-histone targets. Bromodomain binding is dictated by the position of the acetylated lysine as well as interactions with specific residues flanking the acetyl-lysine. PCAF also specifically binds the HIV viral protein Tat on acetylated K50 to regulate its transactivating activity and help induce chromatin remodeling of proviral genes, thereby promoting transcription of viral proteins.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Gene symbol
    KAT2A, MIR658, MIR492, KAT2B
  • Short name
    PCAF bromodomain (492-658 aa), recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    PCAF bromodomain (492-658 aa), H. sapiens Rec.
  • Alternative technique
    rec
Gene info
  • Identity
  • Gene
  • Long gene name
    lysine acetyltransferase 2A
  • Synonyms gene
  • Synonyms gene name
    • GCN5 general control of amino-acid synthesis 5-like 2 (yeast)
    • K(lysine) acetyltransferase 2A
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-12-17
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • GCN5 related N-acetyltransferases
    • ATAC complex
    • SAGA complex
    • Lysine acetyltransferases
    • Bromodomain containing
  • VEGA ID
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    lysine acetyltransferase 2B
  • Synonyms gene
  • Synonyms gene name
    • p300/CBP-associated factor
    • K(lysine) acetyltransferase 2B
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1999-02-10
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • MicroRNA protein coding host genes
    • Bromodomain containing
    • GCN5 related N-acetyltransferases
    • ATAC complex
    • SAGA complex
    • Lysine acetyltransferases
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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