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Available ordering format
Lyophilized
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Immunogen
Purified native plastocyanin from Spinacia oleracea P00289
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunogold (IG)
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Connected products
AS06 202 | anti-cytc6 marker antibody of thylakoid lumen for Chlamydomonas reinhardtiiPlant protein extraction buffer Secondary antibodies
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Recommended dilutions for use
1:2000 with alkaline phosphatase (WB), 1: 100 (IG)
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Molecular weight expected аpparent
10 kDa
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Verified reactivity
Arabidops thaliana, Brassica juncea, Heliantus annuus, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Zea mays
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Possible reactivity
dicots Ricinus communis, Solanum lycopersicum, monocots including Hordeum vulgare, moss Physcomitrella patens
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.
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References
Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.Perera-Garcia et al. (2013). Arabidopsis copper transport protein COPT2 participates in the crosstalk between iron deficiency responses and low phosphate signaling.
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Scientific context
Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.
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Notes
cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing proclin if requested
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Protein number
P00289
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TAIR number
AT1G20340
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