p21 Antibody

• Catalog number
  F51647-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  p21
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IF, ELISA
• Recommended dilutions
  Western blot: 1:1000,Immunofluorescence: 1:10-1:50
• Notes
  Titration of the p21 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This p21 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P38936
• Purity
  Antigen affinity
• Description
  This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
• Immunogen
  A portion of amino acids 105-140 from the human protein was used as the immunogen for this p21 antibody.
• Storage
  Aliquot the p21 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  p21  
• Gene symbol
  PAK4, PAK6, PAK5
• Short name
  Anti-p21
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to p21
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:16059
• Gene
  PAK4
• Long gene name
  p21 (RAC1) activated kinase 4
• Synonyms gene name
  • p21(CDKN1A)-activated kinase 4
  • p21 protein (Cdc42/Rac)-activated kinase 4
• GenBank acession
  • AJ011855
• Locus
  19q13.2
• Discovery year
  2001-07-12
• Entrez gene record
  10298
• Pubmed identfication
  • 9822598
  • 10461188
• VEGA ID
  OTTHUMG00000182800

Gene info

• Identity
  HGNC:16061
• Gene
  PAK6
• Long gene name
  p21 (RAC1) activated kinase 6
• Synonyms gene name
  • p21(CDKN1A)-activated kinase 6
  • p21 protein (Cdc42/Rac)-activated kinase 6
• Synonyms
  • PAK5
• GenBank acession
  • AF276893
• Locus
  15q15.1
• Discovery year
  2001-07-12
• Entrez gene record
  56924
• Pubmed identfication
  • 11278661
• VEGA ID
  OTTHUMG00000129921

Gene info

• Identity
  HGNC:15916
• Gene
  PAK5
• Long gene name
  p21 (RAC1) activated kinase 5
• Synonyms gene
  • PAK7
• Synonyms gene name
  • p21(CDKN1A)-activated kinase 7
  • p21 protein (Cdc42/Rac)-activated kinase 7
  • p21 (RAC1) activated kinase 7
• Synonyms
  • KIAA1264
• GenBank acession
  • AB033090
• Locus
  20p12.2
• Discovery year
  2001-06-21
• Entrez gene record
  57144
• Pubmed identfication
  • 11756552
  • 10574462
• VEGA ID
  OTTHUMG00000031857

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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