PAF Acetylhydrolase (PAF-AH) Inhibitor Screening Kit (Colorimetric)
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Catalog numberK766-100
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PricePlease ask
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Size100 assays
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DescriptionSimple assay to screen/study/characterize PAF Acetylhydrolase Inhibitors
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Summary• Detection: Absorbance (OD 412 nm) • Application: Screening/studying/characterizing potential PAF-AH inhibitors
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Detection MethodAbsorbance (OD 412 nm)
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ApplicationsScreening/studying/characterizing potential PAF-AH inhibitors
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Features Benefits• Rapid & simple • Reliable test for screening potential inhibitors of PAF-AH • Includes PAF-AH Inhibitor (MAFP)
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundPlatelet-Activating Factor (PAF or 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is an important phospholipid mediator, which has diverse biological activities. PAF is synthesized and secreted by a variety of cells such as mast cells, monocytes, macrophages etc. Up-regulated PAF signaling can cause pathological inflammation and also has been found to be responsible for sepsis, shock, and traumatic injury. PAF-Acetylhydrolase (PAF-AH or 1-alkyl-2-acetylglycerophosphocholine esterase or Lipoprotein-associated Phospholipase A2 or Lp-PLA2) (EC 3.1.1.47) hydrolyzes PAF by removing acetyl group at the sn-2 position and converts PAF into biologically inactive form, lyso-PAF. PAF-AH has two forms: extracellular and intracellular that shares some similarities. In humans, PAF-AH deficiency leads to severe asthma, thus development of novel and specific inhibitors of PAF-AH is critical for therapeutic purposes. In BioVision’s PAF-AH Inhibitor Screening Kit, PAF-AH hydrolyzes the acetyl thioester bond at sn-2 position of substrate and free thiols are detected using DTNB. In the presence of PAF-AH inhibitor, the reaction is impeded. The PAF-AH Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be finished in less than 1 hr.
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PropertiesColorimetric assays or detection use UV absorption or enzymatic color reaction.
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Additional descriptionTissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Gene target
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Gene symbolPEX6, PEX2, PCLAF, PLA2G7, PAFAH1B1
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Short namePAF Acetylhydrolase (PAF-AH) Inhibitor Screening Kit (Colorimetric)
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Techniquecolorimetric, colorimetry
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Alternative namePAF Acetylhydrolase (PAF-AH) suppressor detection reagent (colorimetry)
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Alternative techniqueassays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameperoxisomal biogenesis factor 6
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-05-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Peroxins
- AAA ATPases
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameperoxisomal biogenesis factor 2
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Synonyms gene
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Synonyms gene name
- peroxisomal membrane protein 3 (35kD, Zellweger syndrome)
- peroxisomal membrane protein 3, 35kDa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1992-03-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Peroxins
- Ring finger proteins
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namePCNA clamp associated factor
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Synonyms gene
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Synonyms gene name
- KIAA0101
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-06-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namephospholipase A2 group VII
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Synonyms gene name
- phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-20
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Entrez gene record
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Pubmed identfication
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Classification
- Phospholipases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameplatelet activating factor acetylhydrolase 1b regulatory subunit 1
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Synonyms gene
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Synonyms gene name
- platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)
- Miller-Dieker syndrome chromosome region
- platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
- platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)
- platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
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VEGA ID
