MOUSE Anti-p53 (aa20-25):Biotin Antibody

  • Catalog number
    GEN210354
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    p53
  • Other names
    cellular tumor antigen p53 isoform a; Cellular tumor antigen p53; cellular tumor antigen p53; antigen NY-CO-13; phosphoprotein p53; p53 tumor suppressor; transformation-related protein 53; tumor protein p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    p53
  • Gene name synonims
    N/A
  • Other gene names
    TP53; TP53; P53; BCC7; LFS1; TRP53; P53
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2a
  • Clone
    DO-1
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    p53 This item recognises the human p53 tumour suppressor protein, binding to both wild type and mutant forms. p53 is important in multicellular organisms, where it regulates the cell cycle and thus functions as a tumor suppressor that is involved in preventing cancer. DO-1 recognises an epitope at the N-terminal end of p53 between amino acids 20-25.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    Biotin (Purified IgG conjugated to Biotin - liquid)
  • Concentration
    IgG concentration 0.1 miligram per 1 mililiter
  • Storage and shipping
    Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
  • Tested applications
    Flow Cytometry (FACS)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
    p53   aa20-25  
  • Short name
    MOUSE Anti-p53 (aa20-25): Antibody
  • Technique
    Antibody, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, mouses
  • Host
    mouse
  • Label
    Biotin
  • Species
    Mouse, Mouses
  • Alternative name
    Anti-MOUSE ANTI p53 (aa20-25):Biotin
  • Alternative technique
    antibodies, murine
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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