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Synonyms
p38, mitogen-activated protein kinase 13
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Alternative_names
p38, mitogen-activated protein kinase 13
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Description
Involved in a wide variety of cellular processes
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Recombinant
Yes
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Source
Baculovirus (Sf9 insect cells)
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Purity by SDS PAGE
≥80%
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Assay
SDS-PAGE
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Activity Specifications test method
259 nmol phosphate incorporated into MBP protein per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
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Molecular Weight
71.0 kDa
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Storage Temp
-80°C
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Shipping
dry ice
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Shelf Life
12 months
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Concentration
5µg/50 µl
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Appearance
Liquid
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Physical form description
Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
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Background Information
Mitogen-activated protein kinase (MAPK) cascades represent one of the major signal systems used by eukaryotic cells to transduce extracellular signals into cellular responses, and involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The stress-activated protein kinase 4 (SAPK4), or p38 delta, is a member of the MAPK family that are activated by chemical and environmental stresses as well as by proinflammatory cytokines. SAPK4 has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines (1). MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase (2).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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