p300 Antibody (KAT3B)
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Catalog numberR31592
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenp300 (KAT3B)
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, IHC-P
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Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting amounts. Titration of the p300 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis p300 antibodyis to be used only for research purposes and not for diagnostics..
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Gene ID2033
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PurityAntigen affinity
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DescriptionE1A binding protein p300 is a protein that in humans is encoded by the EP300 gene. The gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. p300 appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. p300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
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ImmunogenHuman partial recombinant protein (AA 2065-2414) was used as the immunogen for this KAT3B / p300 antibody.
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StorageAfter reconstitution, the p300 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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LocalizationIntracellular
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolEP300
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Short nameAnti-p300 (KAT3B)
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to p300 (KAT3B)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameE1A binding protein p300
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-07-31
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers ZZ-type
- Lysine acetyltransferases
- Bromodomain containing
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data