p-Rb Antibody (pS249)

  • Catalog number
    F48442-0.4ML
  • Price
    Please ask
  • Size
    0.4 ml
  • Category
    Antibody
  • Concentration
    In 1X PBS, pH 7.4, with 0.09% sodium azide
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    p-Rb (pS249)
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB, IHC, ELISA
  • Recommended dilutions
    Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
  • Notes
    Titration of the p-Rb antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This phospho-p-Rb antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    P06400
  • Purity
    Antigen affinity
  • Description
    RB1 likely acts as a regulator of other genes. It forms a complex with adenovirus E1A and with SV40 large T antigen, acts as a tumor suppressor, and may bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. RB1 is a potent inhibitor of E2F-mediated trans-activation, and also recruits and targets histone methyltransferase SUV39H1 leading to epigenetic transcriptional repression. This protein inhibits the intrinsic kinase activity of TAF1. Defects in RB1 are the cause of childhood cancer retinoblastoma (RB), a congenital malignant tumor that arises from the nuclear layers of the retina. Defects in RB1 are also a cause of bladder cancer and osteogenic sarcoma.
  • Immunogen
    This p-Rb antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS249 of human Retinoblastoma.
  • Storage
    Aliquot the p-Rb antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    p-Rb   pS249  
  • Short name
    Anti-p-Rb (pS249)
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit Ig
  • Alternative name
    Antibodies to p-Rb (pS249)
  • Alternative technique
    antibodies
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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