OxiSelect™ DNA Double-Strand Break (DSB) Staining test kit
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Catalog number
STA-321
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Price
Please ask
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Size
100 assays
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
-20ºC
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SDS or safty data sheet
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Type of reagent
Oxidative Stress/Damage
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Related techniques
Oxidative Stress/ Damage, DNA / RNA Damage and Repair, DNA Double-Strand Break Assay
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Use of the reagent
Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect™ DNA Double-Strand Break Staining test kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.
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Cellular use
Detects phosphorylation of histone H2A.X
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Protocol information
See immunofluorescence in about 3 hours
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Reagent properties
DNA double-strand break inducer included
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,50
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Gene target
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Gene symbol
PRKDC, HLA-DOA
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Short name
OxiSelect™ DNA Double-Strand Break (DSB) Staining test kit
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Technique
dna
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Alternative name
OxiSelect™ Desoxyribonucleic acid Double-Strand Break (DSB) coloring test reagent
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Alternative technique
tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
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