Oxalate, Oxalate oxidase, Biotin conjugated

  • Catalog number
    AS09 519
  • Price
    Please ask
  • Size
    1 ml (10 mg/ml)
  • Available ordering format
    Lyophilized in phosphate buffer saline, labelled with biotin
  • Immunogen
    native oxalate oxidase have been purified from barley seedlings
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Purified IgG
  • How to reconstitute
    For reconstitution please add 1 ml of sterile destilled water.
  • Storage condition
    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
  • Connected products
    AS09 518 | anti-oxalate oxidase antibodyPlant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 1000 to 1: 4000 for techniques listed above
  • Molecular weight expected аpparent
    21 kDa
  • Verified reactivity
    Hordeum vulgare
  • Possible reactivity
    Triticum aestivum, Oryza sativa
  • No reactivity
    n.a.
  • Supplementary information
    antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
  • References
    not available at the moment
  • Scientific context
    Oxalate oxidase (EC=1.2.3.4) belongs to the family of oxidoreductases, and acts on the aldehyde or oxo group of donor with oxygen as acceptor. Those reactions are specific forglyoxylate and dicarboxylate metabolism. Alternative names: oxygen oxidoreductase, aero-oxalo dehydrogenase, oxalic acid oxidase.
  • Notes
    biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidobiotin.
  • Protein number
    Refer to NCBI
  • TAIR number
    Refer to NCBI
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Gene target
  • Short name
    Oxalate, Oxalate oxidase, conjugated
  • Technique
    antibody Conjugates
  • Label
    Biotin
  • Alternative name
    Oxalate, Oxalate oxidase, biotinilated coupled
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