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Available ordering format
Lyophilized in phosphate buffer saline, labelled with biotin
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Immunogen
native oxalate oxidase have been purified from barley seedlings
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Purified IgG
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How to reconstitute
For reconstitution please add 1 ml of sterile destilled water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
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Connected products
AS09 518 | anti-oxalate oxidase antibodyPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 to 1: 4000 for techniques listed above
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Molecular weight expected аpparent
21 kDa
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Verified reactivity
Hordeum vulgare
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Possible reactivity
Triticum aestivum, Oryza sativa
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No reactivity
n.a.
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Supplementary information
antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
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References
not available at the moment
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Scientific context
Oxalate oxidase (EC=1.2.3.4) belongs to the family of oxidoreductases, and acts on the aldehyde or oxo group of donor with oxygen as acceptor. Those reactions are specific forglyoxylate and dicarboxylate metabolism. Alternative names: oxygen oxidoreductase, aero-oxalo dehydrogenase, oxalic acid oxidase.
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Notes
biotin/IgG protein molar ratio (B/P) is approximately 6.6. No foreign proteins are added. Marker used for lebling is N-hydroxysuccinimidobiotin.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated