-
Description
A non-radioactive transcription factor assay with an ELISA format to detect the activation of Oct4 in nuclear extraction and whole lysates.
-
Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Human • Application- Detecting Oct4 in human nuclear extraction and whole lysates
-
Detection Method
Absorbance (450 nm)
-
Species Reactivity
Human
-
Applications
This ELISA kit is used for detecting the Oct4 in human nuclear extraction and whole lysates
-
Sample Type
Nuclear extraction, whole lysates
-
Features Benefits
• A non-radioactive transcription factor assay with an ELISA format. • An easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors.
-
Storage Conditions
-20°C
-
Shipping Conditions
gel pack
-
Shelf life
12 months
-
Background
Pluripotency and differentiation of embryonic stem (ES) cells is controlled by regulatory genes, which are often transcriptional regulators. The roles of these regulators are to repress or activate patterns of gene expression for normal pluripotent cell development and maintenance, and to mediate permanent phenotypic change during stem cell differentiation. Oct4 is one of these important transcription factors specifically expressed in cells with pluripotent identity. For example, a study with Oct4-null mouse ES cells that differentiated into trophectoderm, the lineage that supplies trophoblast cells for the developing placenta, defined the effects of Oct4 on ES cell self-renewal1. Oct4 belongs to the Octamer class of transcription factors because of recognition of an 8-bp consensus ATGCAAAT DNA sequence. Together with Pit and Unc proteins, Oct proteins define the POU (Pit, Oct and Unc) domain that interact with DNA by binding to the 8-bp sequence. Oct4 contains two DNA-binding domains: the 75-amino-acid POU-specific domain (POUS) and the 60-amino-acid carboxy-terminal POU homeodomain (POUHD). Accurate monitoring of the level of activated Oct4 in cells, tissues or animal models is required for investigating signal transduction pathways and other research applications such as drug development. Simple, speedy and high-throughput methods are required for this purpose. The BioVision Oct4 Transcription Factor-Activity Assay kit is a non-radioactive transcription factor assay with an ELISA format. It offers an easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors.
-
-
Test
Biovision supplies other types of Assays as 1.
-
Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
