Occludin Antibody

• Catalog number
  R30159
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Occludin
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the Occludin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Occludin antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q16625
• Purity
  Antigen affinity
• Description
  Occludin is an integral membrane protein that is located at tight junctions. It is a member of a family of proteins containing the highly conserved Marvel domain, which contains 4 transmembrane-helix regions. This gene is mapped to 5q13.2. Occludin regulates TGF-beta receptor type I localization for efficient TGF-beta-dependent dissolution of tight junctions during epithelial-mesenchymal transitions. It is an essential hepatitis C virus (HCV) cell entry factor that is able to render murine cells infectable with HCV glycoproteins. It has been found that Occludin is involved in cell migration and functions to recruit active PI3 kinase to the leading edge, resulting in RAC1 activation and formation of lamellipodia.
• Immunogen
  Human partial recombinant protein (AA 373-522) was used as the immunogen for this Occludin antibody.
• Storage
  After reconstitution, the Occludin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Occludin  
• Gene symbol
  OCLN, OCEL1, MARVELD1
• Short name
  Anti-Occludin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Occludin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:8104
• Gene
  OCLN
• Long gene name
  occludin
• Synonyms
  • PPP1R115
• Synonyms name
  • tight junction protein occludin TM4 minus
  • phosphatase 1, regulatory subunit 115
• GenBank acession
  • U49184
• Locus
  5q13.2
• Discovery year
  1998-01-20
• Entrez gene record
  100506658
• Pubmed identfication
  • 8601611
• RefSeq identity
  • NM_002538
• Classification
  • Protein phosphatase 1 regulatory subunits
  • Tetraspan junctional complex superfamily
  • MARVEL domain containing
• VEGA ID
  OTTHUMG00000099356

Gene info

• Identity
  HGNC:26221
• Gene
  OCEL1
• Long gene name
  occludin/ELL domain containing 1
• Synonyms
  • FLJ22709
• GenBank acession
  • BC029361
• Locus
  19p13.11
• Discovery year
  2006-08-22
• Entrez gene record
  79629
• Pubmed identfication
  • 12477932
• RefSeq identity
  • NM_024578
• VEGA ID
  OTTHUMG00000182726

Gene info

• Identity
  HGNC:28674
• Gene
  MARVELD1
• Long gene name
  MARVEL domain containing 1
• Synonyms gene
  • MRVLDC1
• Synonyms gene name
  • MARVEL (membrane-associating) domain containing 1
• Synonyms
  • MGC4415
  • GB14
  • FLJ23440
  • FLJ22343
  • bA548K23.8
• Synonyms name
  • occludin
• GenBank acession
  • BC004995
• Locus
  10q24.2
• Discovery year
  2004-03-05
• Entrez gene record
  83742
• Pubmed identfication
  • 19364627
  • 21347699
• RefSeq identity
  • NM_031484
• Classification
  • MARVEL domain containing
• VEGA ID
  OTTHUMG00000018865

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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