Nuclear/Cytosol Fractionation Kit
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Catalog number
K266-25
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Price
Please ask
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Size
25 assays
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Description
Isolates nuclear/cytosol fractions w/o untracentrifugation
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Summary
• Species reactivity- Mammalian • Kit size- Conveient sizes (25, 100) • Applications- This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells and tissues The new kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more downstream assays.
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Sample Type
Cell cultures (adherent and suspension), fresh and frozen tissues
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Features Benefits
• Simple procedure; takes less than 2-3 hours • Fast and convenient • The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
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Gene target
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Short name
/Cytosol Fractionation Kit
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Alternative name
Nuclear/cytoplasm Fractionation reagent
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Alternative technique
kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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