NTA-FITC (90%)

• Catalog number
  N925000
• Price
  Please ask
• Size
  10 mg

• Chemical available in other sizes
  Please inquire size and price
• Stock availability
  In Stock
• Cas number
  This product doesn't have CAS Number
• Chemical s molecular weight
  651.64
• Chemical s main applications
  A fluorescent reagent for the detection of proteins having Histidine-tag (his-tag).
• Chemical s category
  Fluorescent Labels & Indicators
• Other name
  N-[6-(2-Biscarboxymethylamino)-1-carboxypentyl]-N’-[5-fluoresceinyl]thiourea
• Chemical s formula
  C31H29N3O11S
• Physical properties
  Orange to Red Solid
• Melting temperature
  >140ºC (dec.)
• Boiling temperature
  No Data Available
• Chemical s soluble in
  Methanol (Slightly)
• Stability conditions
  Light Sensitive, Hygroscopic
• Storage
  Amber Vial, -20°C Freezer, Under Inert Atmosphere
• Chemical s specifications
• Properties
  This trca Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. trca FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  NTA  
• Short name
  NTA-FITC (90%)
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  NTA-fluorecein (90%)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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