KEAP1 Antibody
-
Catalog numberF40464-0.4ML
-
PricePlease ask
-
Size0.4 ml
-
-
CategoryAntibody
-
ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
-
FormAntigen affinity purified
-
ConjugationUnconjugated
-
ClonePolyclonal antibody
-
Recognised antigenKEAP1
-
Host animalRabbit (Oryctolagus cuniculus)
-
ClonalityPolyclonal (rabbit origin)
-
Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
-
Tested applicationsWB, IHC, ELISA
-
Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:50-1:100
-
NotesThe stated application concentrations are suggested starting amounts. Titration of the KEAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
-
Intented useThis KEAP1 antibodyis to be used only for research purposes and not for diagnostics..
-
UniprotQ14145
-
PurityAntigen affinity
-
DescriptionKEAP1 acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome [UniProt]
-
ImmunogenA portion of amino acids 422-449 from the human protein was used as the immunogen for this KEAP1 antibody.
-
StorageStore at 4oC for up to one month. For long term, aliquot the KEAP1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
-
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translationanticorps
-
Gene target
-
Gene symbolKEAP1
-
Short nameAnti-KEAP1
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
IsotypeRabbit Ig
-
Alternative nameAntibodies to KEAP1
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namekelch like ECH associated protein 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2003-09-30
-
Entrez gene record
-
RefSeq identity
-
Classification
- BTB domain containing
- Kelch like
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data