NPC1 Antibody

• Catalog number
  F42992-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  NPC1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  IF, IHC, WB, ELISA
• Recommended dilutions
  Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50
• Notes
  Titration of the NPC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This NPC1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O15118
• Purity
  Antigen affinity
• Description
  This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.
• Immunogen
  A portion of amino acids 591-620 from the human protein was used as the immunogen for this NPC1 antibody.
• Storage
  Aliquot the NPC1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  NPC1  
• Gene symbol
  NPC1
• Short name
  Anti-NPC1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to NPC1
• Alternative technique
  antibodies
• Alternative to gene target
  Niemann-Pick disease, type C1, NPC, NPC1 and IDBG-1600 and ENSG00000141458 and 4864, cholesterol binding, nuclei, Npc1 and IDBG-128877 and ENSMUSG00000024413 and 18145, NPC1 and IDBG-643938 and ENSBTAG00000015195 and 286772

Gene info

• Identity
  HGNC:7897
• Gene
  NPC1
• Long gene name
  NPC intracellular cholesterol transporter 1
• Synonyms gene name
  • Niemann-Pick disease, type C1
• Synonyms
  • SLC65A1
• GenBank acession
  • AF002020
• Locus
  18q11.2
• Discovery year
  1993-04-13
• Entrez gene record
  4864
• Pubmed identfication
  • 8446622
• RefSeq identity
  • NM_000271
• Classification
  • Solute carriers
• VEGA ID
  OTTHUMG00000131873

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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