Flag and His tagging of cytoplasmic and nuclear proteins in baculovirus system

• Catalog number
  B9-50
• Price
  Please ask
• Size
  50 μL

• Description
  Flag and His tagging of cytoplasmic and nuclear proteins in baculovirus system (pVL-FH)
• Produced in
  Spodoptera frugiperda, Trichoplusia ni
• Application
  Protein expression
• Gene number
  Please refer to Genebank
• Delivery
  Shipped under blue ice
• Storage
  Store at plus 4°C
• Technical file
  Please inquire the latest datasheet, protocol, certificate of analysis or SDS
• Other products
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
• Conjugation
  Flag, histidine

• Gene target
  His   tagging   cytoplasmic   proteins   baculovirus  
• Short name
  pVL-FH
• Label
  Flag
• Alternative name
  Flag and histidine tagging on cytoplasmic and nuclear proteins in baculovirus system

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