NME4, human recombinant

• Catalog number
  7826-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D
• Alternative_names
  Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D
• Description
  A ubiquitous enzyme that catalyze transfer of gamma-phosphates via a phosphohistidine intermediate.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is > 15 units/ml, in which one unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.
• Results
  Specific activity is > 15 units/ml
• Unit Definition
  One unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.
• Molecular Weight
  19.6 kDa (176 aa, 33-187 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, and 0.2 M NaCl
• Background Information
  NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NME4  
• Gene symbol
  NME4
• Short name
  NME4, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  NME4, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7852
• Gene
  NME4
• Long gene name
  NME/NM23 nucleoside diphosphate kinase 4
• Synonyms gene name
  • non-metastatic cells 4, protein expressed in
• Synonyms
  • nm23-H4
  • NM23H4
  • NDPKD
• GenBank acession
  • Y07604
• Locus
  16p13.3
• Discovery year
  1996-12-19
• Entrez gene record
  4833
• Pubmed identfication
  • 9099850
  • 19852809
• RefSeq identity
  • NM_005009
• Classification
  • NME/NM23 family
• VEGA ID
  OTTHUMG00000047995

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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