NME1, human recombinant

• Catalog number
  7823-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1.
• Alternative_names
  Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1.
• Description
  A candidate metastasis suppressor gene
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein (determined by LAL method)
• Biological activity
  Specific activity is > 180 unit/ml, in which one unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.
• Results
  Specific activity is > 180 unit/ml
• Unit Definition
  One unit will convert 1.0 µmole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25ºC in a coupled system with PK/LDH.
• Molecular Weight
  17.1 kDa (152 aa, 1-152 aa)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol and 1 mM DTT
• Background Information
  Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas. Recombinant human NME1 was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  NME1  
• Gene symbol
  NME1, NME1-NME2, RMRP
• Short name
  NME1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  NME/NM23 nucleoside diphosphate kinase 1, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  NME/NM23 nucleoside diphosphate kinase 1, AWD and GAAD and NB and NBS and NDKA and NDPK-A and NDPKA and NM23 and NM23-H1, NME1 and IDBG-409263 and ENSG00000239672 and 4830, poly(A) RNA binding, nuclei, Nme1 and IDBG-208565 and ENSMUSG00000037601 and 18102, NME1 and IDBG-638442 and ENSBTAG00000004651 and 404189

Gene info

• Identity
  HGNC:7849
• Gene
  NME1
• Long gene name
  NME/NM23 nucleoside diphosphate kinase 1
• Synonyms gene name
  • non-metastatic cells 1, protein (NM23A) expressed in
• Synonyms
  • NM23
  • NM23-H1
  • NDPKA
• GenBank acession
  • AL360191
  • X17620
• Locus
  17q21.33
• Discovery year
  1991-07-26
• Entrez gene record
  4830
• Pubmed identfication
  • 8270257
  • 19852809
• RefSeq identity
  • NM_000269
• Classification
  • NME/NM23 family
  • SET complex
• VEGA ID
  OTTHUMG00000137474

Gene info

• Identity
  HGNC:33531
• Gene
  NME1-NME2
• Long gene name
  NME1-NME2 readthrough
• Synonyms
  • NM23-LV
  • NMELV
• GenBank acession
  • DQ109675
• Locus
  17q21.33
• Discovery year
  2007-07-31
• Entrez gene record
  654364
• Pubmed identfication
  • 16442775
• VEGA ID
  OTTHUMG00000137475

Gene info

• Identity
  HGNC:10031
• Gene
  RMRP
• Long gene name
  RNA component of mitochondrial RNA processing endoribonuclease
• Synonyms gene
  • CHH
• Synonyms gene name
  • cartilage-hair hypoplasia
• Synonyms
  • RMRPR
  • RRP2
  • NME1
• GenBank acession
  • M29916
• Locus
  9p13.3
• Discovery year
  1989-06-30
• Entrez gene record
  6023
• Pubmed identfication
  • 11207361
  • 1623519
• RefSeq identity
  • NR_003051
• Locus Specific Databases
  • LRG_163

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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