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Other name
NACHT,LRR and PYD domains-containing protein 3,Cold autoinflammatory syndrome 1 protein homolog,Cryopyrin,Mast cell maturation-associated-inducible protein 1,PYRI,Nlrp3,Cias1,Mmig1,Nalp3,Pypaf1
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Immunogen
Recombinant mouse NACHT, LRR and PYD domains-containing protein 3 protein (1-153AA)
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Verified reactivity
Mouse, human
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Verified applications
ELISA,IF
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Raised in
Rabbit
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Clonality
Polyclonal
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Protein number
Q8R4B8
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Gene number
Please refer to GenBank
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NCBI number
Please refer to NCBI
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Purity
Protein G purified, >95%
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Storage recommendation
Aliquot and store at -20°C. Minimize freezing and thawing.
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Use before
1 year
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Shipping requirements
Blue ice
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Estimated production time
3-7 business days
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Supplementary information
As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, beta-amyloid fibers, environmental or industrial particles and nanoparticles, etc. However, it is unclear what constitutes the direct NLRP3 activator. Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed:26098997).
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Notes
For research use only. Not for diagnostic procedures.
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Properties
If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
NLRP3
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Short name
Nlrp3 Polyclonal Antibody
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Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
Unconjugated
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Alternative name
NLR family, pyrin domain containing 3 polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
NLR family, pyrin domain containing 3, NLRP3 and IDBG-107914 and ENSG00000162711 and 114548, peptidoglycan binding, Cytoplasm, Nlrp3 and IDBG-178924 and ENSMUSG00000032691 and 216799, NLRP3 and IDBG-645402 and ENSBTAG00000001273 and 538639
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