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Target antigen
Nitric Oxide Synthase, Brain NOS1
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Clonality
Monoclonal antibody
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Clone
NOS-B1
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, mouse, rat
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Analyses
WB
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Immunogen
Recombinant neuronal NOS fragment(amino acids 1-181) from rat brain.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the Nitric Oxide Synthase, Brain(1181) NOS1 Antibody ( NOSat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Nitric Oxide Synthase, Brain(1181) NOS1 Antibody ( NOS did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Nitric Oxide Synthase 1(NOS1,neuronal NOS,nNOS1) is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. It may be involved in neuronal cell death and damage in neurological illness. nNOS1 localized to the 12q24.2 region of human chromosome 12. It splice variant, expressed in testis, that encodes an NH2-terminal truncated protein of 1098 amino acids. nNOS cDNA clones were shown to contain different 5' terminal exons spliced to a common exon 2. Genomic cloning and sequence analysis demonstrate that the unique exons are positioned within 300 bp of each other but separated from exon 2 by an intron that is at least 20 kb in length. The neuronal isoform of nitric oxide synthase is highly expressed in mammalian skeletal muscle, it suggested a specific role for NOS1 in the local metabolic inhibition of alpha-adrenergic vasoconstriction in active skeletal muscle. The novel gaseous neuromediator nitric oxide is though to play an important role in development and plasticity. Despite this, gene-knockout mice lacking neuronal(Type I) nitric oxide synthase exhibit relatively normal brain development and behavior.
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Related articles
1. Brenman, J. E.; Chao, D. S.; Xia, H.; Aldape, K.; Bredt, D. S. : Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy. Cell 82: 743-752, 1995. 2. Wang, Y.; Goligorsky, M. S.; Lin, M.; Wilcox, J. N.; Marsden, P. A. : A novel, testis-specific mRNA transcript encoding an NH(2)-terminal truncated nitric-oxide synthase. J. Biol. Chem. 272: 11392-11401, 1997. 3. Xie, J.; Roddy, P.; Rife, T. K.; Murad, F.; Young, A. P. : Two closely linked but separable promoters for human neuronal nitric oxide synthase gene transcription. Proc. Nat. Acad. Sci. 92: 1242-1246, 1995. 4. Kharazia, V. N.; Schmidt, H. H. H. W.; Weinberg, R. J. : Type I nitric oxide synthase fully accounts for NADPH-diaphorase in rat striatum, but not cortex. Neuroscience 62: 983-987, 1994.
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Gene Name
NOS1
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Protein Name
Nitric oxide synthase, brain
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Gene Full Name
nitric oxide synthase 1(neuronal)
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Synonyms
NOS|bNOS|nNOS|IHPS1|N-NOS|NC-NOS|Neuronal NOS|Peptidyl-cysteine S-nitrosylase NOS1
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Uniprot ID
Human(P29475), Mouse(Q9Z0J4), Rat(P29476)
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Entrez GeneID
Human(4842), Mouse(18125), Rat(24598)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
