Ni-IDA Spin Columns

• Catalog number
  6567-25
• Price
  Please ask
• Size
  25 columns

• Description
  Columns with Hi-Bind™ Ni QR Agarose Beads for small scale purification of poly-histidine tagged proteins. Protein activity and functional studies rely largely on the availability of purified target proteins. Majority of protein studies are carried out on tagged recombinant proteins expressed in various host organisms. Over 50% of these tagged recombinant proteins are expressed as fusions with poly-histidine purification tags. The small size and the mild conditions utilized during His-tagged protein purification as well as the associated low costs makes this type of fusion the most popular (and in many cases, the first tag of choice). Ni-IDA Spin Columns are ideal for small-scale purification of these poly-histidine-tagged proteins rapidly. Each of these columns is filled with 100 µL of high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562), enabling efficient capture and purification of up to 2.8 mg of poly-histidine tagged proteins per column. The small volume of beads inside the columns is optimal for loading various amounts of sample. At normal expression levels, it permits efficient displacement of non-tagged protein impurities by the more strongly binding poly-histidine tagged targets and improves the purity of the final product. The yield and purity of purification are protein specific and depends on various factors like the expression level, structure, solubility etc. The beads packed in each column deliver as much as 25% higher capacity than Ni-NTA adsorbents while dramatically decreasing purification times due to the ability to perform centrifugation.
• Product Highlights
  Salient Features: Binding Capacity: Upto 2.8 mg poly-histidine tagged proteins per column. Content: 25 Ni-IDA spin columns, each containing 100 µl high performance Hi-Bind™ Ni QR Agarose Beads (Cat. #6562). Bead content: A 50% slurry (e.g., 1 ml of settled resin is equivalent to 2 ml of 50% slurry) in 20% ethanol. Applications: • Ideal for small scale, one – step purification of poly-histidine tagged recombinant proteins under native conditions. • Ideal for small scale, one – step purification of native proteins and peptides that have an affinity for metal ions. • Screening of expression levels in a HTP manner. • Applicable for purification under native and denaturing conditions.
• Storage Temp
  +4°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.

• Gene target
  Ni-IDA   Spin   Columns  
• Short name
  Ni-IDA Spin Columns
• Alternative name
  Ni-IDA Spin Columns

MeSH Data

• Name
  Chromatography
• Concept
  Scope note: Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
• Tree numbers
  • E05.196.181
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Similar products