NFKB p65 Ser276 Antibody Conjugated
NFKB p65 Ser276 Antibody Conjugated is available 7 times from Bioss primary conjugated antibodies labs
- CategoryConjugated Primary Antibodies
- Host OrganismRabbit (Oryctolagus cuniculus)
- Target AntigenNFKB p65 Ser276
- SpecificityThis is a highly specific antibody against NFKB p65 Ser276.
- Modification siteSer276
- ClonePolyclonal antibody
- Concentration1ug per 1ul
- SourceKLH conjugated synthetic phosphopeptide derived from human NFKBp65 around the phosphorylation site of Ser276
- Gene ID number5970
- Swiss ProtQ04206
- CrossreactivityHuman, Mouse, Rat
- Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
- Antigen backgroundNF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
- Purification methodThis antibody was purified via Protein A.
- Synonymsp65; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA
- Gene target
- Alternative techniqueantibodies
bs-3543R-Biotin | NFKB p65(Ser276) Antibody, Biotin Conjugatedsize: 0.1ml | 430.54 USD
- Catalog numberbs-3543R-Biotin
- Price430.54 USD
- Long nameNFKB p65(Ser276) Polyclonal Antibody, Biotin Conjugated
- Tested applicationsWB, IHC-P
- Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500)
- Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
- Excitation EmissionN/A
- PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
- Additional conjugationBiotinylated
- GeneNF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
- French translationanticorps
- Short nameNFKB p65(Ser276) Antibody, Conjugated
- TechniqueAntibody, antibodies against human proteins, antibodies for, antibody Conjugates
- Alternative nameNFKB p65(Ser276) (Antibody to), biotinilated coupled
1. Gene info
- Long gene namenuclear factor kappa B subunit 1
- Synonyms gene name
- nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
- GenBank acession
- Discovery year1991-11-14
- Entrez gene record
- Pubmed identfication
- RefSeq identity
- Ankyrin repeat domain containing
- NF-kappa B complex subunits
- Havana BLAST/BLAT
- ConceptScope note:Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
- Tree numbers
- Qualifiersclassification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics
- PubMedCo-localization of NFkappaB p65 immunoreactivity with TUNEL is observed in scattered photoreceptor cells after 24 h of light exposure and suggests a neuroprotective role in light-induced photoreceptor degeneration.NF-kappa B subunit p65 has critical inhibitory functions during the systemic response to lipopolysaccharide which could be essential in preventing mortality associated with systemic inflammatory response syndromes.p65 NF-kappaB/RelA induces apoptosis of Kupffer cells and inhibition of p65NF-kappaB/RelA attenuates elastase-induced upregulation of proapoptotic pathways and apoptosis in Kupffer cells.Early phase of Theiler murine encephalomyelitis is characterized by an upregulation of c-jun, c-fos, p50, and p65 in susceptible SJL/J mice, probably driving proinflammatory cascade.Data indicate that pre-treatment with paricalcitol has a renoprotective effect in ischemia/reperfusion (I/R) induced acute kidney injury (AKI), possibly by suppressing TLR4-NF-kappaB P65 mediated inflammation.In response to Helicobacter pylori infection, ILK modulates TNF-alpha production and activates nuclear factor kappaB signaling via p65 Ser-536 phosphorylation.NF-kappaB is required for expression of proinflammatory genes in the lean but not in the obese condition. NF-kappaB is required for inhibition of apoptosis in the obese condition, in which proinflammation is enhanced by NF-kappaB inactivation.The anti-inflammatory proteolytic targeting of NF-kappaB p65 by SOCS1 is an active mechanism during lipopolysacchride stimulation of macrophages.
- Gene ontology - Biological process
- Gene ontology - Cellular component
- Gene ontology - Molecular function
- Entrez Gene
bs-3543R-Cy3 | NFKB p65(Ser276) Antibody, Cy3 Conjugatedsize: 0.1ml | 452.68 USD
bs-3543R-Cy5 | NFKB p65(Ser276) Antibody, Cy5 Conjugatedsize: 0.1ml | 452.68 USD
bs-3543R-Cy5.5 | NFKB p65(Ser276) Antibody, Cy5.5 Conjugatedsize: 0.1ml | 452.68 USD
bs-3543R-Cy7 | NFKB p65(Ser276) Antibody, Cy7 Conjugatedsize: 0.1ml | 452.68 USD
bs-3543R-FITC | NFKB p65(Ser276) Antibody, FITC Conjugatedsize: 0.1ml | 430.54 USD
bs-3543R-HRP | NFKB p65(Ser276) Antibody, HRP Conjugatedsize: 0.1ml | 452.68 USD
Supplier:Bioss Primary Unconjugated Antibodies
Supplier:Bioss Primary Unconjugated Antibodies