Neuroserpin, human recombinant
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Catalog number7181-10
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PricePlease ask
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Size10 ug
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SynonymsSerpin I1, Protease inhibitor 12
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Alternative_namesSerpin I1, Protease inhibitor 12
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DescriptionPlays an important role in controlling proteolytic degradation of ECM
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥96%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Activity Specifications test methodDetermined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 ug/ml.
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Biological activityDetermined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 ug/ml.
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Molecular Weight44.8 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionSterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate pH 7.8 and 50 mM NaCl
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background InformationNeuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB). Recombinant human neuroserpin is a 44.8 kDa non-glycosylated protein containing 395 amino-acid residues.
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Amino acid sequenceMTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSERPINI1, SERPINI2
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Short nameNeuroserpin, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameNeuroserpin, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameserpin family I member 1
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-12-20
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Entrez gene record
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Pubmed identfication
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Classification
- Serpin peptidase inhibitors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameserpin family I member 2
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2
- serine (or cysteine) proteinase inhibitor, clade I (pancpin), member 2
- serpin peptidase inhibitor, clade I (pancpin), member 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1995-12-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Serpin peptidase inhibitors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data