Neuroserpin, human recombinant

• Catalog number
  7181-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  Serpin I1, Protease inhibitor 12
• Alternative_names
  Serpin I1, Protease inhibitor 12
• Description
  Plays an important role in controlling proteolytic degradation of ECM
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥96%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 ug/ml.
• Biological activity
  Determined by the dose-dependent stimulation of the proliferation of rat C6 cells using a concentration range of 0.3-0.6 ug/ml.
• Molecular Weight
  44.8 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Phosphate pH 7.8 and 50 mM NaCl
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  Neuroserpin is an inhibitory serpin that is expressed predominantly in central nervous system. Although the physiological target of neuroserpin is still unclear, cumulative evidence suggest that it plays an important role in controlling proteolytic degradation of extracellular matrix (ECM) during synaptogenesis and the subsequent development of neuronal plasticity. In the adult brain, neuroserpin is secreted from the growth cones of neurons in areas where synaptic changes are associated with learning and memory, i.e. cerebral cortex, hippocampus, and amygdala. The neuroprotective role of neuroserpin has been demonstrated in transgenic mice lacking neuroserpin expression. The deficiency of neuroserpin in these mice was associated with motor neuron disease characterized by axonal degradation. In humans, defects in neuroserpin, caused by point mutations in the neuroserpin gene, underlie a hereditary disorder called the familial encephalopathy with neuroserpin inclusion bodies (FENIB). Recombinant human neuroserpin is a 44.8 kDa non-glycosylated protein containing 395 amino-acid residues.
• Amino acid sequence
  MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Neuroserpin  
• Gene symbol
  SERPINI1, SERPINI2
• Short name
  Neuroserpin, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Neuroserpin, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8943
• Gene
  SERPINI1
• Long gene name
  serpin family I member 1
• Synonyms gene
  • PI12
• Synonyms gene name
  • serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1
• Synonyms name
  • neuroserpin
• GenBank acession
  • Z81326
• Locus
  3q26.1
• Discovery year
  1995-12-20
• Entrez gene record
  5274
• Pubmed identfication
  • 24172014
• Classification
  • Serpin peptidase inhibitors
• VEGA ID
  OTTHUMG00000158450

Gene info

• Identity
  HGNC:8945
• Gene
  SERPINI2
• Long gene name
  serpin family I member 2
• Synonyms gene
  • PI14
• Synonyms gene name
  • serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 2
  • serine (or cysteine) proteinase inhibitor, clade I (pancpin), member 2
  • serpin peptidase inhibitor, clade I (pancpin), member 2
• Synonyms
  • TSA2004
  • MEPI
• Synonyms name
  • myoepithelium-derived serine proteinase inhibitor
  • pancpin
• GenBank acession
  • AB006423
• Locus
  3q26.1
• Discovery year
  1995-12-20
• Entrez gene record
  5276
• Pubmed identfication
  • 9624529
  • 24172014
• RefSeq identity
  • NM_006217
• Classification
  • Serpin peptidase inhibitors
• VEGA ID
  OTTHUMG00000158231

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products