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Also known as
Neugrin, Neurite Outgrowth Associated
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Other names
neugrin; Neugrin; neugrin; spinal cord-derived protein FI58G; mesenchymal stem cell protein DSC92; neurite outgrowth associated protein; neurite outgrowth-associated protein; neugrin, neurite outgrowth associated; Mesenchymal stem cell protein DSC92; Neurite outgrowth-associated protein; Spinal cord-derived protein FI58G
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
NGRN; NGRN; DSC92; FI58G
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Gene name synonims
NGRN; NGRN; DSC92; FI58G
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Other gene names
NGRN; NGRN; DSC92; FI58G
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG2b
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Clone
9j66
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes human NGRN.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.05% sodium azide.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
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Tested applications
Western Blot (WB)
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Description
For cells, cell lines and tissues in culture till half confluency.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
