NEDD8 Antibody

• Catalog number
  bs-3812R
• Price
  Please ask
• Size
  0.1ml

• Long name
  NEDD8 Primary Polyclonal Antibody
• Also known as
  NEDD8 PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  NEDD8
• Specificity
  This is a highly specific antibody against NEDD8.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human NEDD8
• Gene ID number
  4738
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Human, Mouse, Rat
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the target antigen
  Ubiquitin like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12 and Hub1. Proteins of the second class include Parkin, RAD23 and DSK2, are designated ubiquitin domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog NEDD8 are activated by the E1 ubiquitin activating enzyme UBA2, that forms isopeptide linkages between thio esters. NEDD8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3 ubiquitin ligase complex called the Skp1 /Cul1/Cdc53 F box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  NED8; NEDD 8; Neddylin; Neural precursor cell expressed developmentally down regulated 8; Neural precursor cell expressed developmentally down regulated gene 8; Rub1; Ubiquitin like protein Nedd 8; Ubiquitin like protein Nedd8.
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  NEDD8  
• Gene symbol
  NEDD8-MDP1, NEDD8
• Short name
  Anti-NEDD8
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  NEDD8 Polyclonal Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:39551
• Gene
  NEDD8-MDP1
• Long gene name
  NEDD8-MDP1 readthrough
• Locus
  14q12
• Discovery year
  2011-02-21
• Entrez gene record
  100528064
• RefSeq identity
  • NM_001199823.1
• VEGA ID
  OTTHUMG00000167107

Gene info

• Identity
  HGNC:7732
• Gene
  NEDD8
• Long gene name
  NEDD8 ubiquitin like modifier
• Synonyms gene name
  • neural precursor cell expressed, developmentally down-regulated 8
• Synonyms
  • Nedd-8
• GenBank acession
  • D23662
• Locus
  14q12
• Discovery year
  1994-01-21
• Entrez gene record
  4738
• Pubmed identfication
  • 9353319
• RefSeq identity
  • NM_006156
• VEGA ID
  OTTHUMG00000029325

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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